5T4 Recombinant Rabbit Monoclonal Antibody [SU34-05]
cat.: ET1608-37
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | SU34-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human 5T4 aa 381-420 / 420. |
| Positive control: | MCF-7 cell lysates, SK-Br-3 cell lysates. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IP |
1:500-1:2,000 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q13641 Human | Q9Z0L0 Mouse | Q5PQV5 Rat |
| Alternative names: | 5T4 5T4 oncofetal antigen 5T4 oncofetal trophoblast glycoprotein 5T4 oncotrophoblast glycoprotein 5T4AG AW495680 M6P1 TPBG TPBG_HUMAN Trophoblast glycoprotein |
Images
|
Fig1:
Western blot analysis of 5T4 on MCF-7 cell lysates with Rabbit anti-5T4 antibody (ET1608-37) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 46 kDa Observed band size: 75 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-37) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of 5T4 on SK-Br-3 cell lysates with Rabbit anti-5T4 antibody (ET1608-37) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 46 kDa Observed band size: 75 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-37) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.