5T4 Recombinant Rabbit Monoclonal Antibody [SU34-05]
cat.: ET1608-37
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SU34-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human 5T4 aa 381-420 / 420. |
| Positive control: | MCF7 cell lysate, Human lung tissue lysate, SK-Br-3 cell lysates, human placenta tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IP IHC-P |
1:500-1:2,000 Use at an assay dependent concentration. 1:1,000 |
| Uniprot #: | SwissProt: Q13641 Human | Q9Z0L0 Mouse | Q5PQV5 Rat |
| Alternative names: | 5T4 5T4 oncofetal antigen 5T4 oncofetal trophoblast glycoprotein 5T4 oncotrophoblast glycoprotein 5T4AG AW495680 M6P1 TPBG TPBG_HUMAN Trophoblast glycoprotein |
Images
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Fig1:
Western blot analysis of 5T4 on different lysates with Rabbit anti-5T4 antibody (ET1608-37) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (20 µg/Lane) Lane 2: Human lung tissue lysate (40 µg/Lane) Predicted band size: 46 kDa Observed band size: 75 kDa Exposure time: Lane 1: 25 seconds; Lane 2: 2 minutes 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-37) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of 5T4 on SK-Br-3 cell lysates with Rabbit anti-5T4 antibody (ET1608-37) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 46 kDa Observed band size: 75 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-37) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-5T4 antibody (ET1608-37) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-37) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.