beta 2 Microglobulin Recombinant Rabbit Monoclonal Antibody [SU35-05]
cat.: ET1608-45
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | SU35-05 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 14 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human beta 2 Microglobulin. |
| Positive control: | Mouse spleen tissue lysate, mouse liver tissue lysate, rat spleen tissue lysate, rat liver tissue lysate, Raji cell lysate, U937 cell lysate, Hela, A431. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000-1:5,000 1:50-1:200 |
| Uniprot #: | SwissProt: P61769 Human | P01887 Mouse | P07151 Rat |
| Alternative names: | B2M B2MG_HUMAN Beta 2 microglobin Beta 2 microglobulin Beta 2 microglobulin precursor Beta chain of mhc class 1 proteins Beta chain of MHC class I molecules Beta-2-microglobulin form pI 5.3 CDABP0092 Hdcma22p IMD43 |
Images
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Fig1:
Western blot analysis of beta 2 Microglobulin on different lysates with Rabbit anti-beta 2 Microglobulin antibody (ET1608-45) at 1/5,000 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat spleen tissue lysate Lane 4: Rat liver tissue lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 14 kDa Observed band size: 12 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-45) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of beta 2 Microglobulin on different lysates with Rabbit anti-beta 2 Microglobulin antibody (ET1608-45) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-beta 2 Microglobulin KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 12 kDa Observed band size: 12 kDa Exposure time: 62 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-45) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Western blot analysis of beta 2 Microglobulin on different lysates with Rabbit anti-beta 2 Microglobulin antibody (ET1608-45) at 1/500 dilution. Lane 1: Raji cell lysate Lane 2: U937 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-45) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig4: ICC staining of beta 2 Microglobulin in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-45, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig5: ICC staining of beta 2 Microglobulin in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-45, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.