CD90 / THY1 Recombinant Rabbit Monoclonal Antibody [SU35-07]
cat.: ET1608-46
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SU35-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 18 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CD90 aa 50-99 / 161. |
| Positive control: | Human brain tissue lysate, Rat brain tissue lysate, human brain tissue, human breast cancer tissue, rat brain tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P04216 Human | P01830 Rat |
| Alternative names: | CD7 CD90 CD90 antigen CDw90 FLJ33325 MGC128895 T25 Theta antigen Thy 1 Thy 1 cell surface antigen Thy 1 membrane glycoprotein Thy 1 T cell antigen Thy 1.2 Thy-1 antigen Thy-1 membrane glycoprotein Thy1 Thy1 antigen Thy1 T cell antigen Thy1.1 Thy1.2 THY1_HUMAN Thymus cell antigen 1, theta |
Images
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Fig1:
Western blot analysis of CD90 / THY1 on different lysates with Rabbit anti-CD90 / THY1 antibody (ET1608-46) at 1/2,000 dilution. Lane 1: Human brain tissue lysate (20 µg/Lane) Lane 2: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 18 kDa Observed band size: 25 kDa Exposure time: 2 minutes 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-46) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-CD90 / THY1 antibody (ET1608-46) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-46) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-CD90 / THY1 antibody (ET1608-46) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-46) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-CD90 / THY1 antibody (ET1608-46) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-46) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.