CaMKⅡ Recombinant Rabbit Monoclonal Antibody [SU03-57]
cat.: ET1608-47
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, IHC-Fr
Clonality: Monoclonal
Clone number: SU03-57
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 54 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human CaMKⅡ aa 201-250 / 478.
Positive control: SH-SY-5Y cell lysate, PC-12 cell lysate, SHG-44 cell lysate, Hela, PC-12, SHG-44, rat brain tissue, rat cerebellum tissue, mouse brain tissue, mouse cerebellum tissue.
Subcellular location: Cytoplasm, Sarcoplasmic reticulum membrane, Cell membrane, Cell junction.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  IHC-Fr
1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:200
1:100
Uniprot #: SwissProt: Q13554 Human | Q13555 Human | Q13557 Human | Q9UQM7 Human | P11798 Mouse | P28652 Mouse | Q6PHZ2 Mouse | Q923T9 Mouse | P08413 Rat | P11275 Rat | P11730 Rat | P15791 Rat
Alternative names: Calcium/calmodulin dependent protein kinase II alpha Calcium/calmodulin dependent protein kinase II beta Calcium/calmodulin dependent protein kinase II delta Calcium/calmodulin dependent protein kinase II gamma Calcium/calmodulin-dependent protein kinase type II subunit alpha CaM kinase II alpha CaM kinase II CaM kinase II beta CaM kinase II delta CaM kinase II gamma CaM kinase II subunit alpha CaMK-II subunit alpha CAMK2 Camk2a CAMK2B CAMK2D CAMK2G CAMKA KCC2A_HUMAN
Images
ET1608-47_1.jpg Fig1: Western blot analysis of CaMKⅡ on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-47, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SH-SY-5Y cell lysate
Lane 2: PC-12 cell lysate
Lane 3: SHG-44 cell lysate
ET1608-47_2.jpg Fig2: ICC staining of CaMKⅡ in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-47, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1608-47_3.jpg Fig3: ICC staining of CaMKⅡ in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-47, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1608-47_4.jpg Fig4: ICC staining of CaMKⅡ in SHG-44 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-47, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1608-47_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CaMKⅡ antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-47, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1608-47_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-CaMKⅡ antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-47, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1608-47_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CaMKⅡ antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-47, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1608-47_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-CaMKⅡ antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-47, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1608-47_9.jpg Fig9: Immunofluorescence analysis of frozen mouse hippocampus tissue labeling CaMKⅡ with Rabbit anti-CaMKⅡ antibody (ET1608-47).

The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody ((ET1608-47, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.
ET1608-47_10.jpg Fig10: Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling CaMKⅡ with Rabbit anti-CaMKⅡ antibody (ET1608-47).

The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody ((ET1608-47, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.