CD31 Recombinant Rabbit Monoclonal Antibody [SU03-59]
cat.: ET1608-48
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, FC, IP
Clonality: Monoclonal
Clone number: SU03-59
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 83 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human CD31 aa 689-738 / 738.
Positive control: THP-1 cell lysate, human placenta tissue lysate, human tonsil tissue, human kidney tissue, human uterus tissue, THP-1.
Subcellular location: Cell junction. Cell membrane. Membrane.
Recommended Dilutions:
  WB
  IHC-P
  FC
  IP

1:1,000-1:2,000
1:50-1:200
1:50-1:100
Use at an assay dependent concentration.
Uniprot #: SwissProt: P16284 Human
Alternative names: Adhesion molecule CD31 CD31 antigen CD31 EndoCAM EndoCAM FLJ34100 FLJ58394 GPIIA GPIIA' PECA1 PECA1_HUMAN Pecam 1 PECAM 1 CD31 EndoCAM PECAM PECAM-1 Pecam1 Platelet and endothelial cell adhesion molecule 1 Platelet endothelial cell adhesion molecule Platelet/endothelial cell adhesion molecule 1
Images
ET1608-48_1.jpg Fig1: Western blot analysis of CD31 on different lysates with Rabbit anti-CD31 antibody (ET1608-48) at 1/500 dilution.

Lane 1: THP-1 cell lysate (10 µg/Lane)
Lane 2: Human placenta tissue lysate (20 µg/Lane)

Predicted band size: 83 kDa
Observed band size: 130 kDa

Exposure time: 30 seconds;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-48) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET1608-48_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD31 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-48, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1608-48_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD31 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-48, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1608-48_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CD31 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-48, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1608-48_5.jpg Fig5: Flow cytometric analysis of CD31 was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1608-48, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.