Phospho-RSK1 p90 (T359 + S363) Recombinant Rabbit Monoclonal Antibody [SU03-65]
cat.: ET1608-53
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, IP
Clonality: Monoclonal
Clone number: SU03-65
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 83 kDa
Isotype: IgG
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Thr359 and Ser363 of Human RSK1 p90 aa 341-390 / 735.
Positive control: MCF-7 cell lysate, A431 cell lysate, 293 cell lysate, A431, HepG2, 293, human breast carcinoma tissue, mouse liver tissue.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  IP
1:1,000-1:2,000
1:50-1:200
1:50-1:200
Use at an assay dependent concentration.
Uniprot #: SwissProt: Q15418 Human | P18653 Mouse | Q63531 Rat
Alternative names: 90 kDa ribosomal protein S6 kinase 1 dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 dJ590P13.1 EC 2.7.11.1 HU 1 HU1 KS6A1_HUMAN MAP kinase activated protein kinase 1a MAP kinase-activated protein kinase 1a MAPK-activated protein kinase 1a MAPKAP kinase 1a MAPKAPK-1a MAPKAPK1A MGC79981 Mitogen-activated protein kinase-activated protein kinase 1A OTTHUMP00000004113 p90 RSK1 p90-RSK 1 p90rsk p90RSK1 p90S6K pp90RSK1 Ribosomal protein S6 kinase 90kD 1 Ribosomal protein S6 kinase 90kD polypeptide 1 Ribosomal protein S6 kinase 90kDa polypeptide 1 Ribosomal protein S6 kinase alpha 1 Ribosomal protein S6 kinase alpha-1 Ribosomal protein S6 kinase polypeptide 1 Ribosomal S6 kinase 1 RPS6K1 alpha rps6ka Rps6ka1 RSK 1 RSK 1 p90 RSK RSK-1 RSK1 S6K alpha 1 S6K-alpha-1
Images
ET1608-53_1.jpg Fig1: Western blot analysis of Phospho-RSK1 p90 (T359 + S363) on different lysates with Rabbit anti-Phospho-RSK1 p90 (T359 + S363) antibody (ET1608-53) at 1/500 dilution.

Lane 1: MCF-7 cell lysate
Lane 2: A431 cell lysate
Lane 3: 293 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 83 kDa
Observed band size: 83 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-53) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET1608-53_2.jpg Fig2: ICC staining of Phospho-RSK1 p90 (T359 + S363) in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-53, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1608-53_3.jpg Fig3: ICC staining of Phospho-RSK1 p90 (T359 + S363) in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-53, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1608-53_4.jpg Fig4: ICC staining of Phospho-RSK1 p90 (T359 + S363) in 293 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-53, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1608-53_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-RSK1 p90 (T359 + S363) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-53, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1608-53_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Phospho-RSK1 p90 (T359 + S363) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-53, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.