ASK1 Recombinant Rabbit Monoclonal Antibody [SU36-06]
cat.: ET1608-54
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SU36-06 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 155 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human ASK1. |
| Positive control: | BxPC-3 cell lysate, Neuro-2a cell lysate, human brain tissue lysate, mouse brain tissue lysate, human lung carcinoma tissue, human pancreas tissue, mouse pancreas tissue, human breast carcinoma tissue. |
| Subcellular location: | Endoplasmic reticulum, Cytoplasm. |
| Recommended Dilutions:
WB IF-Tissue IHC-P |
1:500-1:2,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: Q99683 Human | O35099 Mouse |
| Alternative names: | Apoptosis signal regulating kinase 1 Apoptosis signal-regulating kinase 1 ASK 1 ASK-1 ASK1 M3K5 M3K5_HUMAN MAP/ERK kinase kinase 5 MAP3K5 MAPK/ERK kinase kinase 5 MAPKKK5 MEK kinase 5 MEKK 5 MEKK5 Mitogen activated protein kinase kinase kinase 5 Mitogen-activated protein kinase kinase kinase 5 |
Images
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Fig1:
Western blot analysis of ASK1 on different lysates with Rabbit anti-ASK1 antibody (ET1608-54) at 1/1,000 dilution. Lane 1: BxPC-3 cell lysate Lane 2: Neuro-2a cell lysate Lane 3: Human brain tissue lysate Lane 4: Mouse brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 155 kDa Observed band size: 150 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-54) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-ASK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-54, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-ASK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-54, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-ASK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-54, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ASK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-54, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.