PARP1 Recombinant Rabbit Monoclonal Antibody [SU03-68]
cat.: ET1608-56
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC
Clonality: Monoclonal
Clone number: SU03-68
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 113 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal human PARP1.
Positive control: A549 cell lysate, Jurkat cell lysate, Hela cell lysate, HeLa, human spleen tissue, mouse large intestine tissue, human breast carcinoma tissue, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate.
Subcellular location: Nucleus, nucleolus, chromosome.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
1:2,000
1:500
1:50-1:800
1:500-1:4,000
1:1,000
Uniprot #: SwissProt: P09874 Human | P11103 Mouse | P27008 Rat
Alternative names: ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) ADP ribosyltransferase diphtheria toxin like 1 ADP ribosyltransferase NAD(+) ADPRT 1 ADPRT ADPRT1 ARTD1 msPARP NAD(+) ADP ribosyltransferase 1 NAD(+) ADP-ribosyltransferase 1 pADPRT 1 pADPRT1 PARP 1 PARP PARP-1 PARP1 PARP1_HUMAN Poly (ADP ribose) polymerase 1 poly (ADP ribose) polymerase family, member 1 Poly [ADP-ribose] polymerase 1 Poly(ADP ribose) polymerase poly(ADP ribose) synthetase poly(ADP ribosyl)transferase Poly[ADP ribose] synthetase 1 Poly[ADP-ribose] synthase 1 PPOL
Images
ET1608-56_1.jpg Fig1: Western blot analysis of PARP1 on different lysates with Rabbit anti-PARP1 antibody (ET1608-56) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution.

Lane 1: HeLa cell lysate (15 µg/Lane)
Lane 2: Jurkat cell lysate (15 µg/Lane)
Lane 3: NIH/3T3 cell lysate (15 µg/Lane)
Lane 4: C2C12 cell lysate (15 µg/Lane)
Lane 5: C6 cell lysate (15 µg/Lane)
Lane 6: PC-12 cell lysate (15 µg/Lane)

Predicted band size: 113 kDa
Observed band size: 113 kDa

Exposure time: 6 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-56) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
ET1608-56_2.jpg Fig2: Western blot analysis of PARP1 on different lysates with Rabbit anti-PARP1 antibody (ET1608-56) at 1/2,000 dilution.

Lane 1: HeLa whole cell lysate
Lane 2: HeLa treated with 1μM staurosporine for 3 hours whole cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 113 kDa
Observed band size: 113/28 kDa

Exposure time: 1 minute 9 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-56) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
ET1608-56_3.jpg Fig3: All lanes: Western blot analysis of PARP with anti-PARP1 antibody[SU03-68] (ET1608-56) at 1:500 dilution.
Lane 1/2: Wild-type Hela whole cell lysate (10 µg).
Lane 3/4: PARP knockdown Hela whole cell lysate (10 µg).

ET1608-56 was shown to specifically react with PARP in wild-type Hela cells. Weakened bands were observed when PARP knockdown samples were tested. Wild-type and PARP knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1608-56, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET1608-56_4.jpg Fig4: Immunocytochemistry analysis of HeLa cells labeling PARP1 with Rabbit anti-PARP1 antibody (ET1608-56) at 1/500 dilution.

Cells were fixed in 100% methanol for 5 minutes, blocked with 2% normal goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PARP1 antibody (ET1608-56) at 1/500 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, green) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/800 dilution.
ET1608-56_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-PARP1 antibody (ET1608-56) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-56) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1608-56_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-PARP1 antibody (ET1608-56) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-56) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1608-56_7.jpg Fig7: Flow cytometric analysis of HeLa cells labeling PARP1.

Cells were fixed and permeabilized. Then stained with the primary antibody (ET1608-56, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
ET1608-56_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-PARP1 antibody (ET1608-56) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-56) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1608-56_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-PARP1 antibody (ET1608-56) at 1/4,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-56) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1608-56_10.jpg Fig10: Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-PARP1 antibody (ET1608-56) at 1/4,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-56) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1608-56_11.jpg Fig11: Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-PARP1 antibody (ET1608-56) at 1/4,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-56) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1608-56_12.jpg Fig12: Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-PARP1 antibody (ET1608-56) at 1/4,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-56) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.