p16INK4a Recombinant Rabbit Monoclonal Antibody [SU0702]
cat.: ET1608-62
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | SU0702 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 16 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human p16INK4a aa 107-156 / 156. |
| Positive control: | 293 cell lysate, SiHa cell lysate, HepG2, Hela, PC-3M, human colon carcinoma tissue, human cervical carcinoma tissue, human ovary carcinoma tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IP |
1:1,000 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P42771 Human |
| Alternative names: | CCM2 CDK4 inhibitor p16 INK4 CDK4I CDKN2 CDKN2A Cell cycle negative regulator beta CMM2 Cyclin dependent kinase 4 inhibitor A Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) Cyclin Dependent Kinase Inhibitor 2A Cyclin dependent kinase inhibitor 2A isoform 4 Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 Cyclin dependent kinase inhibitor p16 INK4 INK4A MLM MTS1 Multiple tumor suppressor 1 p14 p16 P16INK4 p16INK4a p19 p19Arf TP16 |
Images
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Fig1:
Western blot analysis of p16INK4a on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-62, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysate Lane 2: SiHa cell lysate |
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Fig2:
Western blot analysis of p16INK4a on different lysates with Rabbit anti-p16INK4a antibody (ET1608-62) at 1/1,000 dilution. Lane 1: HeLa-si NT cell lysate Lane 2: HeLa-si p16INK4a cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-62) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.