Anti-Myosin light chain kinase antibody [SU40-06]
cat.: ET1608-68
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC-P, FC
Clonality: Monoclonal
Clone number: SU40-06
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 210/203/205/197/202/110/80 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal human MYLK.
Positive control: Human lung tissue lysates, rat lung tissue lysates, Hela, L6, NIH/3T3, SH-SY5Y, rat smooth muscle tissue, human colon carcinoma tissue, mouse smooth muscle tissue.
Subcellular location: Cytoplasm, Cell projection, Cleavage furrow.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  FC

1:1,000-1:10,000
1:100-1:500
1:100-1:500
1:50-1:100
Uniprot #: SwissProt: Q15746 Human | Q6PDN3 Mouse
Unigene: 203004 Rat
Alternative names: deglutamylated form DKFZp686I10125 EC 2.7.11.18 FLJ12216 Kinase related protein Kinase-related protein KRP MLCK MLCK1 MLCK108 MLCK210 MSTP083 MYLK MYLK_HUMAN MYLK1 Myosin light chain kinase Myosin light polypeptide kinase OTTHUMP00000180642 OTTHUMP00000180643 smMLCK smooth muscle Smooth muscle myosin light chain kinase Telokin
Images
ET1608-68_1.jpg Fig1: Western blot analysis of Myosin light chain kinase on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-68, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1608-68_2.jpg Fig2: Western blot analysis of Myosin light chain kinase on rat lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-68, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1608-68_3.jpg Fig3: ICC staining of Myosin light chain kinase in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-68, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1608-68_4.jpg Fig4: ICC staining of Myosin light chain kinase in L6 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-68, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1608-68_5.jpg Fig5: ICC staining of Myosin light chain kinase in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-68, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1608-68_6.jpg Fig6: ICC staining of Myosin light chain kinase in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-68, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1608-68_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded rat smooth muscle tissue using anti-Myosin light chain kinase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-68, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1608-68_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Myosin light chain kinase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-68, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1608-68_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-Myosin light chain kinase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-68, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1608-68_10.jpg Fig10: Flow cytometric analysis of Myosin light chain kinase was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1608-68, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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