Caspase-1 Recombinant Rabbit Monoclonal Antibody [SU40-07]
cat.: ET1608-69
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | SU40-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Caspase-1 aa 213-404 / 404. |
| Positive control: | THP-1 cell lysate, HCT 116 cell lysate, HL-60 cell lysate, RAW264.7 cell lysate, Mouse spleen lysate, Rat spleen lysate, U937 cell lysates, PC-12, HL-60, THP-1. |
| Subcellular location: | Cytoplasm, Cell membrane. |
| Recommended Dilutions:
WB FC IF-Cell |
1:1,000-1:100,000 1:1,000 1:100 |
| Uniprot #: | SwissProt: P29466 Human | P29452 Mouse | P43527 Rat |
| Alternative names: | Caspase1 CASP-1 CASP1 CASP1_HUMAN Caspase1 Caspase 1 Caspase-1 subunit p10 ICE IL-1 beta-converting enzyme IL-1BC IL1 beta converting enzyme IL1B convertase Interleukin 1 beta convertase Interleukin 1B converting enzyme Interleukin-1 beta convertase Interleukin-1 beta-converting enzyme p45 |
Images
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Fig1:
Western blot analysis of Caspase-1 on different lysates with Rabbit anti-Caspase-1 antibody (ET1608-69) at 1/100,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HCT 116 cell lysate Lane 3: HL-60 cell lysate Lane 4: RAW264.7 cell lysate Lane 5: Mouse spleen lysate Lane 6: Rat spleen lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-69) at 1/100,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Caspase-1 on U937 cell lysates with Rabbit anti-Caspase-1 antibody (ET1608-69) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-69) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunocytochemistry analysis of PC-12 cells labeling Caspase-1 with Rabbit anti-Caspase-1 antibody (ET1608-69) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Caspase-1 antibody (ET1608-69) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Flow cytometric analysis of PC-12 cells labeling Caspase-1. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1608-69, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig5:
Immunocytochemistry analysis of HL-60 cells labeling Caspase-1 with Rabbit anti-Caspase-1 antibody (ET1608-69) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Caspase-1 antibody (ET1608-69) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig6:
Immunocytochemistry analysis of THP-1 cells labeling Caspase-1 with Rabbit anti-Caspase-1 antibody (ET1608-69) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Caspase-1 antibody (ET1608-69) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.