M-CSF Recombinant Rabbit Monoclonal Antibody [SU0413]
cat.: ET1609-1
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IHC-P, IF-Cell, IP
Clonality: Monoclonal
Clone number: SU0413
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 60 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human M-CSF aa 56-105 / 554.
Positive control: Jurkat cell lysate, THP-1 cell lysate, mouse spleen tissue lysate, mouse colon tissue lysate, A431, HepG2, Hela, human kidney tissue, mouse colon tissue, human lung tissue, human liver tissue.
Subcellular location: Membrane, Secreted, extracellular space, nuclear body.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  IP
1:500-1:1,000
1:50-1:200
1:50-1:2,000
Use at an assay dependent concentration.
Uniprot #: SwissProt: P09603 Human | P07141 Mouse
Alternative names: Colony stimulating factor 1 (macrophage) Colony stimulating factor 1 Colony stimulating factor macrophage specific CSF 1 CSF-1 CSF1 CSF1_HUMAN Csfm Lanimostim M CSF M-CSF Macrophage Colony Stimulating Factor 1 Macrophage colony stimulating factor MCSF MGC31930 Processed macrophage colony-stimulating factor 1
Images
ET1609-1_1.jpg Fig1: Western blot analysis of M-CSF on different lysates with Rabbit anti-M-CSF antibody (ET1609-1) at 1/2,000 dilution.

Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: THP-1 cell lysate (20 µg/Lane)

Predicted band size: 60 kDa
Observed band size: 60 kDa

Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-1) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1609-1_2.jpg Fig2: Western blot analysis of M-CSF on different lysates with Rabbit anti-M-CSF antibody (ET1609-1) at 1/2,000 dilution.

Lane 1: Mouse spleen tissue lysate (40 µg/Lane)
Lane 2: Mouse colon tissue lysate (40 µg/Lane)

Predicted band size: 60 kDa
Observed band size: 60 kDa

Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-1) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1609-1_3.jpg Fig3: ICC staining of M-CSF in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-1, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1609-1_4.jpg Fig4: ICC staining of M-CSF in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-1, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1609-1_5.jpg Fig5: ICC staining of M-CSF in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-1, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1609-1_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-M-CSF antibody (ET1609-1) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-1) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1609-1_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-M-CSF antibody (ET1609-1) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-1) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1609-1_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-M-CSF antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-1, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1609-1_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-M-CSF antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-1, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.