ET1609-10

YB1 Recombinant Rabbit Monoclonal Antibody [ST0432]

cat.: ET1609-10

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Cell, IF-Tissue, IHC-P, FC, IP
Clonality:	Monoclonal
Clone number:	ST0432

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 36 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within Human YB1 aa 275-324 / 324.
Positive control:	HeLa cell lysate, Jurkat cell lysate, Hela, MCF-7, HepG2, human kidney tissue, mouse colon tissue, mouse stomach tissue, human prostate carcinoma tissue.
Subcellular location:	Cytoplasm, Nucleus, Cytoplasmic granule, Secreted.
Recommended Dilutions: WB IF-Cell IF-Tissue IHC-P FC IP	1:1,000-1:5,000 1:100-1:500 1:100-1:500 1:50-1:200 1:50-1:100 Use at an assay dependent concentration.
Uniprot #:	SwissProt: P67809 Human \| P62960 Mouse \| P62961 Rat
Alternative names:	BP 8 CBF-A CCAAT binding transcription factor I subunit A CCAAT-binding transcription factor I subunit A CSDA2 CSDB DBPB DNA binding protein B DNA-binding protein B EFI-A Enhancer factor I subunit A MDR NF1 MGC104858 MGC110976 MGC117250 NSEP 1 NSEP1 Nuclease sensitive element binding protein 1 Nuclease-sensitive element-binding protein 1 p50 Q15905 Y-box binding protein 1 Y-box transcription factor Y-box-binding protein 1 YB 1 YB-1 YBOX1_HUMAN YBX 1 ybx1

Images

	Fig1: Western blot analysis of YB1 on different lysates with Rabbit anti-YB1 antibody (ET1609-10) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 36 kDa Observed band size: 50 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-10) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
	Fig2: ICC staining YB1(1/200) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig3: ICC staining YB1(1/50) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

	Fig4: ICC staining YB1(1/50) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-YB1 antibody at 1/200. Counter stained with hematoxylin.
	Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-YB1 antibody at 1/200. Counter stained with hematoxylin.
	Fig7: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-YB1 antibody at 1/200. Counter stained with hematoxylin.

Fig8: Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-YB1 antibody (ET1609-10) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1609-10) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig9: Flow cytometric analysis of Hela cells with YB1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.