Phospho-p53 (S376) Recombinant Rabbit Monoclonal Antibody [ST0440]
cat.: ET1609-14
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | ST0440 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 53 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Ser376 of Human p53 aa 344-393 / 393. |
| Positive control: | HEK-293 treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hours whole cell lysate, RAW264.7 treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hours whole cell lysate. |
| Subcellular location: | Cytoplasm, Nucleus, Endoplasmic reticulum, Mitochondrion matrix. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P04637 Human |
| Alternative names: | Antigen NY-CO-13 BCC7 Cellular tumor antigen p53 FLJ92943 LFS1 Mutant tumor protein 53 p53 p53 tumor suppressor P53_HUMAN Phosphoprotein p53 Tp53 Transformation related protein 53 TRP53 Tumor protein 53 Tumor protein p53 Tumor suppressor p53 |
Images
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Fig1:
Western blot analysis of Phospho-p53 (S376) on different lysates with Rabbit anti-Phospho-p53 (S376) antibody (ET1609-14) at 1/1,000 dilution. Lane 1: HEK-293 whole cell lysate Lane 2: HEK-293 treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hours whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-14) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Phospho-p53 (S376) on different lysates with Rabbit anti-Phospho-p53 (S376) antibody (ET1609-14) at 1/1,000 dilution. Lane 1: RAW264.7 whole cell lysate Lane 2: RAW264.7 treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hours whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-14) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.