Anti-Phospho-Src(Y419) antibody [ST0800]
cat.: ET1609-15
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, FC
Clonality: Monoclonal
Clone number: ST0800
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 60 kDa
Isotype: IgG
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Tyr419 of Human Src aa 401-450 / 536.
Positive control: A549 cell lysates.
Subcellular location: Cell membrane, Mitochondrion inner membrane, Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  FC

1:1,000
1:50-1:100
Uniprot #: SwissProt: P12931 Human | P05480 Mouse | Q9WUD9 Rat
Alternative names: ASV Avian sarcoma virus c SRC CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA cSrc EC 2.7.10.2 Neuronal CSRC tyrosine specific protein kinase Neuronal SRC Oncogene SRC OTTHUMP00000174476 OTTHUMP00000174477 p60 Src p60-Src p60Src pp60c src pp60c-src pp60csrc Proto oncogene tyrosine protein kinase Src Proto-oncogene c-Src Proto-oncogene tyrosine-protein kinase Src Protooncogene SRC Protooncogene SRC Rous sarcoma Src SRC Oncogene SRC proto oncogene non receptor tyrosine kinase SRC_HUMAN SRC1 Tyrosine kinase pp60c src Tyrosine protein kinase SRC 1 Tyrosine protein kinase SRC1 v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian) v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian
Images
ET1609-15_1.jpg Fig1: Western blot analysis of Phospho-Src(Y419) on A549 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-15, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1609-15_2.jpg Fig2: Flow cytometric analysis of Hela cells with Phospho-Src(Y419) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.