Phospho-Src (Y419) Recombinant Rabbit Monoclonal Antibody [ST0800]
cat.: ET1609-15
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | ST0800 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 60 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Tyr419 of Human Src aa 401-450 / 536. |
| Positive control: | A549 cell lysates, HepG2 cell lysates, Hela, A549, SH-SY5Y. |
| Subcellular location: | Cell membrane, Mitochondrion inner membrane, Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB FC IF-Cell |
1:1,000 1:50-1:100 1:50 |
| Uniprot #: | SwissProt: P12931 Human | P05480 Mouse | Q9WUD9 Rat |
| Alternative names: | ASV Avian sarcoma virus c SRC CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA cSrc EC 2.7.10.2 Neuronal CSRC tyrosine specific protein kinase Neuronal SRC Oncogene SRC OTTHUMP00000174476 OTTHUMP00000174477 p60 Src p60-Src p60Src pp60c src pp60c-src pp60csrc Proto oncogene tyrosine protein kinase Src Proto-oncogene c-Src Proto-oncogene tyrosine-protein kinase Src Protooncogene SRC Protooncogene SRC Rous sarcoma Src SRC Oncogene SRC proto oncogene non receptor tyrosine kinase SRC_HUMAN SRC1 Tyrosine kinase pp60c src Tyrosine protein kinase SRC 1 Tyrosine protein kinase SRC1 v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian) v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian |
Images
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Fig1: Western blot analysis of Phospho-Src (Y419) on A549 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-15, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Phospho-Src(Y419) on HepG2 cell lysates. Lane 1: HepG2 cells, whole cell lysate, 10ug/lane Lane 2/3: HepG2 cells treated with 50 uM pervanadate for 10 minutes, whole cell lysates, 10ug/lane Lane 4: HepG2 cells treated with 50 uM pervanadate for 10 minutes, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-Src(Y419) antibody (ET1609-15) at 1/500 dilution. Anti-Hsp90 beta antibody (ET1605-56) at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution. Predicted band size: 60 kDa Observed band size: 60 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 30 seconds |
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Fig3: Flow cytometric analysis of Hela cells with Phospho-Src(Y419) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
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Fig4:
Immunocytochemistry analysis of A549 cells labeling Phospho-Src (Y419) with Rabbit anti-Phospho-Src (Y419) antibody (ET1609-15) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Phospho-Src (Y419) antibody (ET1609-15) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig5:
Immunocytochemistry analysis of SH-SY5Y cells labeling Phospho-Src (Y419) with Rabbit anti-Phospho-Src (Y419) antibody (ET1609-15) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Phospho-Src (Y419) antibody (ET1609-15) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.