Histone H4 (tri methyl K20) Recombinant Rabbit Monoclonal Antibody [ST044-07]
cat.: ET1609-16
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: ST044-07
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 11 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Histone H4 aa 1-50 / 103.
Positive control: Human liver tissue, human kidney tissue, mouse liver tissue, mouse colon tissue, mouse kidney tissue.
Subcellular location: Nucleus, Chromosome.
Recommended Dilutions:
  WB
  IHC-P

1:1,000
1:50-1:800
Uniprot #: SwissProt: P62805 Human | P62806 Mouse | P62804 Rat
Alternative names: Histone gene cluster 1, H4A Histone gene cluster 2, H4 dJ160A22.1 dJ160A22.2 dJ221C16.1 dJ221C16.9 FO108 H4 H4 histone family, member A H4 histone family, member B H4 histone family, member C H4 histone family, member D H4 histone family, member E H4 histone family, member G H4 histone family, member H H4 histone family, member I H4 histone family, member J H4 histone family, member K H4 histone family, member M H4 histone family, member N H4 histone, family 2 H4/A H4/B H4/C H4/D H4/E H4/G H4/H H4/I H4/J H4/K H4/M H4/N H4/O H4/p H4_HUMAN H4F2 H4F2iii H4F2iv H4FA H4FB H4FC H4FD H4FE H4FG H4FH H4FI H4FJ H4FK HIST1 cluster, H4A HIST1 cluster, H4B HIST1 cluster, H4D HIST2H4 Hist4 cluster, H4 Hist4h4 histone 1, H4a histone 1, H4c histone 1, H4d histone 1, H4f histone 1, H4h histone 1, H4i histone 1, H4j histone 1, H4k histone 1, H4l histone 2, H4a histone 2, H4b ......
Images
ET1609-16_1.jpg Fig1: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Histone H4 (tri methyl K20) antibody at 1/200. Counter stained with hematoxylin.
ET1609-16_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Histone H4 (tri methyl K20) antibody (ET1609-16) at 1/800 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-16) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1609-16_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Histone H4 (tri methyl K20) antibody at 1/200. Counter stained with hematoxylin.
ET1609-16_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H4 (tri methyl K20) antibody. Counter stained with hematoxylin.
ET1609-16_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Histone H4 (tri methyl K20) antibody at 1/200. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.