Anti-Phospho-GATA3 (S308) antibody [ST44-09]
cat.: ET1609-17
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, IP
Clonality: Monoclonal
Clone number: ST44-09
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 48 kDa
Isotype: IgG
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser308 of Human GATA3 aa 281-330 / 443.
Positive control: Human skin tissue lysate, Jurkat cell lysate, human breast carcinoma tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  IP

1:1,000-1:2,000
1:50-1:200
Use at an assay dependent concentration.
Uniprot #: SwissProt: P23771 Human
Alternative names: GATA 3 GATA binding factor 3 GATA binding protein 3 GATA-binding factor 3 Gata3 GATA3_HUMAN HDR HDRS MGC2346 MGC5199 MGC5445 Trans acting T cell specific transcription factor GATA 3 Trans-acting T-cell-specific transcription factor GATA-3
Images
ET1609-17_1.jpg Fig1: Western blot analysis of Phospho-GATA3 (S308) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-17, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: human skin tissue lysate
Lane 2: Jurkat cell lysate
ET1609-17_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-GATA3 (S308) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-17, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.