Androgen receptor Recombinant Rabbit Monoclonal Antibody [ST0453]
cat.: ET1609-18
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | ST0453 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 99 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Androgen receptor aa 595-644 / 644 (P10275-3). |
| Positive control: | 22RV1 cell lysates, PC-3M, MCF-7. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: P10275 Human |
| Alternative names: | AIS ANDR_HUMAN Androgen nuclear receptor variant 2 Androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease) Androgen receptor androgen receptor splice variant 4b AR AR8 DHTR Dihydro testosterone receptor Dihydrotestosterone receptor (DHTR) Dihydrotestosterone receptor HUMARA HYSP1 KD Kennedy disease (KD) NR3C4 Nuclear receptor subfamily 3 group C member 4 (NR3C4) Nuclear receptor subfamily 3 group C member 4 SBMA SMAX1 Spinal and bulbar muscular atrophy (SBMA) Spinal and bulbar muscular atrophy Testicular Feminization (TFM) TFM |
Images
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Fig1:
Western blot analysis of Androgen receptor on 22RV1 cell lysates with Rabbit anti-Androgen receptor antibody (ET1609-18) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 99 kDa Observed band size: 75 kDa Exposure time: 1 minute 2 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-18) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of Androgen receptor in PC-3M cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-18, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of Androgen receptor in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-18, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.