Androgen receptor Recombinant Rabbit Monoclonal Antibody [ST0453]
cat.: ET1609-18
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: ST0453
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 99 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Androgen receptor aa 595-644 / 644 (P10275-3).
Positive control: 22RV1 cell lysates.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB

1:500-1:1,000
Uniprot #: SwissProt: P10275 Human
Alternative names: AIS ANDR_HUMAN Androgen nuclear receptor variant 2 Androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease) Androgen receptor androgen receptor splice variant 4b AR AR8 DHTR Dihydro testosterone receptor Dihydrotestosterone receptor (DHTR) Dihydrotestosterone receptor HUMARA HYSP1 KD Kennedy disease (KD) NR3C4 Nuclear receptor subfamily 3 group C member 4 (NR3C4) Nuclear receptor subfamily 3 group C member 4 SBMA SMAX1 Spinal and bulbar muscular atrophy (SBMA) Spinal and bulbar muscular atrophy Testicular Feminization (TFM) TFM
Images
ET1609-18_1.jpg Fig1: Western blot analysis of Androgen receptor on 22RV1 cell lysates with Rabbit anti-Androgen receptor antibody (ET1609-18) at 1/1,000 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 99 kDa
Observed band size: 75 kDa

Exposure time: 1 minute 2 seconds; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-18) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.