Stathmin 1 Recombinant Rabbit Monoclonal Antibody [SS0453]
cat.: ET1609-20
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | SS0453 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 18 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Stathmin 1 aa 100-149 / 149. |
| Positive control: | PC-12, SH-SY-5Y, Hela, human tonsil tissue, human breast carcinoma tissue, mouse testis tissue, mouse spinal cord tissue, mouse brain tissue. |
| Subcellular location: | Cytoplasm, Cytoskeleton, Microtubule. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC IP |
1:1,000-1:2,000 1:50-1:500 1:50-1:500 1:50-1:200 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P16949 Human | P54227 Mouse | P13668 Rat |
| Alternative names: | C1orf215 Lag LAP 18 LAP18 Leukemia associated phosphoprotein p18 Leukemia-associated phosphoprotein p18 Metablastin Oncoprotein 18 OP 18 Op18 p18 p19 Phosphoprotein 19 Phosphoprotein p19 pp17 pp19 PR22 Pr22 protein Prosolin Protein Pr22 SMN Stathmin Stathmin1 STMN 1 Stmn1 STMN1_HUMAN |
Images
|
Fig1: ICC staining Stathmin 1 (1/50) in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig2: ICC staining Stathmin 1 (1/50) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: ICC staining Stathmin 1 (1/50) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Stathmin 1 antibody at 1/200 dilution. Counter stained with hematoxylin. |
|
Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Stathmin 1 antibody at 1/200 dilution. Counter stained with hematoxylin. |
|
Fig6: Immunohistochemical analysis of paraffin-embedded mouse spinal cord tissue using anti-Stathmin 1 antibody at 1/200 dilution. Counter stained with hematoxylin. |
|
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Stathmin 1 antibody at 1/200 dilution. Counter stained with hematoxylin. |
|
Fig8: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Stathmin 1 antibody at 1/200 dilution. Counter stained with hematoxylin. |
|
Fig9: Flow cytometric analysis of Hela cells with Stathmin 1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
|
Fig10:
Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling Stathmin 1 with Rabbit anti-Stathmin 1 antibody (ET1609-20) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1609-20, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.