Aurora A Recombinant Rabbit Monoclonal Antibody [ST46-07]
cat.: ET1609-22
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | ST46-07 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Aurora A aa 1-50 / 403. |
| Positive control: | K562 cell lysates, SKOV-3, Hela, NIH/3T3, rat testis tissue. |
| Subcellular location: | Cytoplasm, Cytoskeleton, Nucleus, Cell projection, Microtubule. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:2,000 1:50-1:100 1:100-1:500 1:50-1:100 |
| Uniprot #: | SwissProt: O14965 Human | P97477 Mouse | P59241 Rat |
| Alternative names: | AIK ARK-1 ARK1 AURA Aurka Aurora 2 Aurora A Aurora kinase A Aurora-related kinase 1 Aurora/IPL1 like kinase Aurora/IPL1-related kinase 1 AURORA2 Breast tumor-amplified kinase BTAK hARK1 IAK IPL1 related kinase MGC34538 OTTHUMP00000031340 OTTHUMP00000031341 OTTHUMP00000031342 OTTHUMP00000031343 OTTHUMP00000031344 OTTHUMP00000031345 OTTHUMP00000166071 OTTHUMP00000166072 PPP1R47 Protein phosphatase 1, regulatory subunit 47 Serine/threonine kinase 15 Serine/threonine kinase 6 Serine/threonine-protein kinase 15 Serine/threonine-protein kinase 6 Serine/threonine-protein kinase aurora-A STK15 STK6 STK6_HUMAN STK7 |
Images
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Fig1: ICC staining of Aurora A in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-22, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig2: ICC staining of Aurora A in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-22, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of Aurora A in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-22, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Aurora A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-22, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Flow cytometric analysis of Aurora A was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1609-22, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig6:
Western blot analysis of Aurora A on different lysates with Rabbit anti-Aurora A antibody (ET1609-22) at 1/1,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: K-562（Human chronic myeloid leukemia cells） cell lysate Lane 3: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 4: HeLa treated with 100ng/mL Nocodazole for 18 hours cell lysate Lane 5: NIH/3T3 (Mouse fibroblast) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1609-22, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 45.8 kDa Observed band size: 48 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.