ASH2L Recombinant Rabbit Monoclonal Antibody [ST47-01]
cat.: ET1609-24
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P
Clonality: Monoclonal
Clone number: ST47-01
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 69 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human ASH2L aa 533-628 / 628.
Positive control: PC-12 cell lysate, Hela cell lysate, 293T cell lysate, PC12, MCF-7, CRC, mouse testis tissue, human colon tissue, mouse ovary tissue, mouse placenta tissue, rat testis tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P

1:1,000-1:5,000
1:200-1:500
1:200-1:500
1:200-1:500
Uniprot #: SwissProt: Q9UBL3 Human | Q91X20 Mouse
Unigene: 219095 Rat
Alternative names: ASH 2 Ash2 (absent small or homeotic) like Ash2 (absent, small, or homeotic) like (Drosophila) ASH2 ASH2 LIKE ASH2 like protein ASH2-like protein Ash2l ASH2L_HUMAN ASH2L1 ASH2L2 Bre 2 Bre2 Discs 2-like Drosophila absent, small, or homeotic Drosophila ASH2-like Set1/Ash2 histone methyltransferase complex subunit ASH2
Images
ET1609-24_1.jpg Fig1: Western blot analysis of ASH2L on different lysates with Rabbit anti-ASH2L antibody (ET1609-24) at 1/500 dilution.

Lane 1: PC-12 cell lysate
Lane 2: Hela cell lysate
Lane 3: 293T cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 69 kDa
Observed band size: 75 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-24) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET1609-24_2.jpg Fig2: ICC staining ASH2L (1/500) in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1609-24_3.jpg Fig3: ICC staining ASH2L (1/500) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1609-24_4.jpg Fig4: ICC staining ASH2L (1/500) in CRC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1609-24_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ASH2L antibody at 1/200 dilution. Counter stained with hematoxylin.
ET1609-24_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-ASH2L antibody (ET1609-24) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-24) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1609-24_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-ASH2L antibody at 1/200 dilution. Counter stained with hematoxylin.
ET1609-24_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-ASH2L antibody at 1/50 dilution. Counter stained with hematoxylin.
ET1609-24_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-ASH2L antibody (ET1609-24) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-24) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.