ASH2L Recombinant Rabbit Monoclonal Antibody [ST47-01]
cat.: ET1609-24
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | ST47-01 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 69 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ASH2L aa 533-628 / 628. |
| Positive control: | PC-12 cell lysate, Hela cell lysate, 293T cell lysate, PC12, MCF-7, CRC, mouse testis tissue, human colon tissue, mouse ovary tissue, mouse placenta tissue, rat testis tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P |
1:1,000-1:5,000 1:200-1:500 1:200-1:500 1:200-1:500 |
| Uniprot #: | SwissProt: Q9UBL3 Human | Q91X20 Mouse Unigene: 219095 Rat |
| Alternative names: | ASH 2 Ash2 (absent small or homeotic) like Ash2 (absent, small, or homeotic) like (Drosophila) ASH2 ASH2 LIKE ASH2 like protein ASH2-like protein Ash2l ASH2L_HUMAN ASH2L1 ASH2L2 Bre 2 Bre2 Discs 2-like Drosophila absent, small, or homeotic Drosophila ASH2-like Set1/Ash2 histone methyltransferase complex subunit ASH2 |
Images
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Fig1:
Western blot analysis of ASH2L on different lysates with Rabbit anti-ASH2L antibody (ET1609-24) at 1/500 dilution. Lane 1: PC-12 cell lysate Lane 2: Hela cell lysate Lane 3: 293T cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 69 kDa Observed band size: 75 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-24) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining ASH2L (1/500) in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining ASH2L (1/500) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining ASH2L (1/500) in CRC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ASH2L antibody at 1/200 dilution. Counter stained with hematoxylin. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-ASH2L antibody (ET1609-24) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-24) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-ASH2L antibody at 1/200 dilution. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-ASH2L antibody at 1/50 dilution. Counter stained with hematoxylin. |
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Fig9:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-ASH2L antibody (ET1609-24) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-24) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.