Anti-ASH2L antibody [ST47-01]
cat.: ET1609-24
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC-P
Clonality: Monoclonal
Clone number: ST47-01
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A purified.
Molecular weight: 69 kDa
Isotype: IgG
Immunogen: recombinant protein
Positive control: PC12, MCF-7, CRC, mouse ovary tissue, mouse placenta tissue, mouse testis tissue, mouse colon tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P

1:1,000-1:5,000
1:200-1:500
1:200-1:500
Uniprot #: Q9UBL3(Human) Q91X20(Mouse) Unigene:219095(Rat)
Alternative names: ASH 2 antibody Ash2 (absent small or homeotic) like antibody Ash2 (absent, small, or homeotic) like (Drosophila) antibody ASH2 antibody ASH2 LIKE antibody ASH2 like protein antibody ASH2-like protein antibody Ash2l antibody ASH2L_HUMAN antibody ASH2L1 antibody ASH2L2 antibody Bre 2 antibody Bre2 antibody Discs 2-like antibody Drosophila absent, small, or homeotic antibody Drosophila ASH2-like antibody Set1/Ash2 histone methyltransferase complex subunit ASH2 antibody
Images
ET1609-24_1.jpg Fig1: Western blot analysis of ASH2L on different lysates using anti-ASH2L antibody at 1/1,000 dilution.
Positive control:
Lane 1: PC12
Lane 2: 293T
ET1609-24_2.jpg Fig2: ICC staining ASH2L in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1609-24_3.jpg Fig3: ICC staining ASH2L in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1609-24_4.jpg Fig4: ICC staining ASH2L in CRC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1609-24_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ASH2L antibody. Counter stained with hematoxylin.
ET1609-24_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-ASH2L antibody. Counter stained with hematoxylin.
ET1609-24_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-ASH2L antibody. Counter stained with hematoxylin.
ET1609-24_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-ASH2L antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.