Anti-MAP1LC3A antibody [ST47-03]
cat.: ET1609-26
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC-P, IP, FC
Clonality: Monoclonal
Clone number: ST47-03
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 14 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human MAP1LC3A aa 80-121.
Positive control: SHG-44 cell lysate, mouse brain tissue lysate, mouse liver tissue lysate, mouse skeletal muscle tissue lysate, Hela, PC-12, HUVEC, human liver tissue, mouse liver tissue, mouse brain tissue, SH-SY5Y.
Subcellular location: Cytoplasm, Endomembrane system, Cytoplasmic vesicle.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  FC
  IP

1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:100
Use at an assay dependent concentration.
Uniprot #: SwissProt: Q9H492 Human | Q91VR7 Mouse | Q6XVN8 Rat
Alternative names: ATG8E Autophagy-related protein LC3 A Autophagy-related ubiquitin-like modifier LC3 A LC3 LC3A MAP1 light chain 3 like protein 1 MAP1 light chain 3-like protein 1 MAP1A/1B light chain 3 A MAP1A/MAP1B LC3 A MAP1A/MAP1B light chain 3 A MAP1ALC3 MAP1BLC3 Map1lc3a Microtubule associated proteins 1A/1B light chain 3 Microtubule-associated protein 1 light chain 3 alpha Microtubule-associated proteins 1A and 1B, light chain 3 Microtubule-associated proteins 1A/1B light chain 3A MLP3A_HUMAN
Images
ET1609-26_1.jpg Fig1: Western blot analysis of MAP1LC3A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-26, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SHG-44 cell lysate
Lane 2: mouse brain tissue lysate
Lane 3: mouse liver tissue lysate
Lane 4: mouse skeletal muscle tissue lysate
ET1609-26_2.jpg Fig2: ICC staining of MAP1LC3A in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-26, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1609-26_3.jpg Fig3: ICC staining of MAP1LC3A in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-26, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1609-26_4.jpg Fig4: ICC staining of MAP1LC3A in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-26, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1609-26_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MAP1LC3A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-26, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1609-26_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-MAP1LC3A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-26, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1609-26_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MAP1LC3A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-26, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1609-26_8.jpg Fig8: Flow cytometric analysis of MAP1LC3A was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1609-26, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.