MAP1LC3A Recombinant Rabbit Monoclonal Antibody [ST47-03]
cat.: ET1609-26
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | ST47-03 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 14 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human MAP1LC3A aa 81-121 / 121. |
| Positive control: | HeLa treated with 50μM Chloroquine for 18 hours cell lysate, C2C12 cell lysate, C2C12 treated with 50μM Chloroquine for 18 hours cell lysate, C6 cell lysate, C6 treated with 50μM Chloroquine for 18 hours cell lysate, SH-SY5Y, mouse liver tissue, human liver tissue, mouse brain tissue, SH-SY5Y. |
| Subcellular location: | Cytoplasm, Endomembrane system, Cytoplasmic vesicle, Microtubule, Membrane. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC IP |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:200 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q9H492 Human | Q91VR7 Mouse | Q6XVN8 Rat |
| Alternative names: | ATG8E Autophagy-related protein LC3 A Autophagy-related ubiquitin-like modifier LC3 A LC3 LC3A MAP1 light chain 3 like protein 1 MAP1 light chain 3-like protein 1 MAP1A/1B light chain 3 A MAP1A/MAP1B LC3 A MAP1A/MAP1B light chain 3 A MAP1ALC3 MAP1BLC3 Map1lc3a Microtubule associated proteins 1A/1B light chain 3 Microtubule-associated protein 1 light chain 3 alpha Microtubule-associated proteins 1A and 1B, light chain 3 Microtubule-associated proteins 1A/1B light chain 3A MLP3A_HUMAN |
Images
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Fig1:
Western blot analysis of MAP1LC3A on different lysates with Rabbit anti-MAP1LC3A antibody (ET1609-26) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 50μM Chloroquine for 18 hours cell lysate Lane 3: C2C12 cell lysate Lane 4: C2C12 treated with 50μM Chloroquine for 18 hours cell lysate Lane 5: C6 cell lysate Lane 6: C6 treated with 50μM Chloroquine for 18 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 14 kDa Observed band size: 14/16 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-26) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of SH-SY5Y cells labeling MAP1LC3A with Rabbit anti-MAP1LC3A antibody (ET1609-26) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MAP1LC3A antibody (ET1609-26) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-MAP1LC3A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-26, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-MAP1LC3A antibody (ET1609-26) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-26) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-MAP1LC3A antibody (ET1609-26) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-26) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Flow cytometric analysis of MAP1LC3A was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1609-26, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red). |
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