Anti-Rab5 antibody [ST47-04]
cat.: ET1609-27
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: ST47-04
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 24 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Rab5 aa 166-215 / 215.
Positive control: MCF-7, Hela, mouse brain tissue, mouse prostate tissue.
Subcellular location: Cell membrane, Cytoplasm, Cell projection, Membrane.
Recommended Dilutions:
  WB
  IHC-P

1:500-1:1,000
1:50-1:200
Uniprot #: SwissProt: P20339 Human | Q9CQD1 Mouse
Unigene: 44477 Rat
Alternative names: RAB 5 RAB 5A RAB5A RAB5A member RAS oncogene family RAB5A_HUMAN RAS associated protein RAB5A Ras related protein Rab 5A Ras-related protein Rab-5A
Images
ET1609-27_1.jpg Fig1: Western blot analysis of Rab5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-27, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: MCF-7 cell lysate
ET1609-27_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Rab5 antibody. Counter stained with hematoxylin.
ET1609-27_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Rab5 antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.