Hexokinase 1 Recombinant Rabbit Monoclonal Antibody [ST47-05]
cat.: ET1609-28
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | ST47-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 102 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Hexokinase 1 aa 100-140. |
| Positive control: | MCF7 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, Hela cell lysate, 293 cell lysate, HepG2 cell lysate, C2C12, MCF7, human kidney tissue, mouse kidney tissue, rat kidney tissue. |
| Subcellular location: | Mitochondrion outer membrane, Cytoplasm, Membrane, Mitochondrion. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC IP |
1:500-1:2,000 1:100-1:500 1:200 1:1,000 1:1,000 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P19367 Human | P17710 Mouse | P05708 Rat |
| Alternative names: | BB404130 Brain form hexokinase dea DrHXK1 EC 2.7.1.1 Glycolytic enzyme HEXOKIN hexokinase I Hexokinase PI Hexokinase type I Hexokinase, tumor isozyme Hexokinase-1 Hexokinase-A HK I HK1 HK1 tb Hk1-s HK1-ta HK1-tb HK1-tc HKD HKI HMSNR HXK1 HXK1_HUMAN im:7148527 mHk1-s wu:fc09d08 wu:fc16e02 wu:fc21e02 wu:fq14b11 zgc:55790 zgc:77618 |
Images
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Fig1:
Western blot analysis of Hexokinase 1 on different lysates with Rabbit anti-Hexokinase 1 antibody (ET1609-28) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: C2C12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 102 kDa Observed band size: 102 kDa Exposure time: 60 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-28) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Hexokinase 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-28, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: 293 cell lysate Lane 3: MCF7 cell lysate Lane 4: HepG2 cell lysate |
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Fig3:
Immunocytochemistry analysis of C2C12 cells labeling Hexokinase 1 with Rabbit anti-Hexokinase 1 antibody (ET1609-28) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Hexokinase 1 antibody (ET1609-28) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Immunocytochemistry analysis of MCF7 cells labeling Hexokinase 1 with Rabbit anti-Hexokinase 1 antibody (ET1609-28) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Hexokinase 1 antibody (ET1609-28) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Hexokinase 1 antibody (ET1609-28) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-28) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Hexokinase 1 antibody (ET1609-28) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-28) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Hexokinase 1 antibody (ET1609-28) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-28) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Flow cytometric analysis of MCF7 cells labeling Hexokinase 1. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1609-28, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.