PI 3 Kinase p85 beta Recombinant Rabbit Monoclonal Antibody [ST04-77]
cat.: ET1609-30
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | ST04-77 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 82 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PI 3 Kinase p85 beta aa 136-185 / 728. |
| Positive control: | Raji cell lysate, Hela cell lysate, MCF-7 cell lysate, U937 cell lysate, PC-12 cell lysates, Hela, SHG-44, rat testis tissue, rat brain tissue, NIH/3T3 cell lysates. |
| Subcellular location: | Cytosol, nucleus |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC IP |
1:1,000-1:2,000 1:50-1:200 1:50-1:200 1:50-1:200 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: O00459 Human | O08908 Mouse | Q63788 Rat |
| Alternative names: | p85 p85 beta P85B P85B_HUMAN Phosphatidylinositol 3 kinase Phosphatidylinositol 3 kinase regulatory beta subunit Phosphatidylinositol 3 kinase regulatory subunit beta Phosphatidylinositol 3 kinase regulatory subunit polypeptide 2 Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 2 (p85 beta) Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta Phosphatidylinositol 3-kinase regulatory subunit beta Phosphoinositide 3 kinase regulatory subunit 2 (beta) Phosphoinositide 3 kinase regulatory subunit 2 Phosphoinositide 3 kinase regulatory subunit polypeptide 2 (p85 beta) Phosphoinositide 3 kinase regulatory subunit polypeptide 2 Phosphoinositide 3 kinase, regulatory subunit 2 (beta) Phosphoinositide 3 kinase, regulatory subunit 2 (p85 beta) PI3 kinase p85 beta subunit PI3 kinase p85 subunit beta PI3-kinase regulatory subunit beta PI3-kinase subunit p85-beta PI3K PI3K regulatory subunit beta PIK3R 2 PIK3R2 PtdIns 3 kinase p...... |
Images
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Fig1:
Western blot analysis of PI 3 Kinase p85 beta on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-30, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Raji cell lysate Lane 2: Hela cell lysate Lane 3: MCF-7 cell lysate Lane 4: U937 cell lysate |
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Fig2:
Western blot analysis of PI 3 Kinase p85 beta on PC-12 cell lysates with Rabbit anti-PI 3 Kinase p85 beta antibody (ET1609-30) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 82 kDa Observed band size: 82 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-30) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig3: ICC staining of PI 3 Kinase p85 beta in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-30, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: ICC staining of PI 3 Kinase p85 beta in SHG-44 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-30, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-PI 3 Kinase p85 beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-30, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PI 3 Kinase p85 beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-30, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7: Flow cytometric analysis of PI 3 Kinase p85 beta was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1609-30, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red). |
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Fig8:
Western blot analysis of PI 3 Kinase p85 beta on NIH/3T3 cell lysates with Rabbit anti-PI 3 Kinase p85 beta antibody (ET1609-30) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 82 kDa Observed band size: 82 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-30) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.