Dnmt3a Recombinant Rabbit Monoclonal Antibody [ST04-78]
cat.: ET1609-31
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | ST04-78 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 102 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Dnmt3a aa 600-640. |
| Positive control: | SH-SY5Y cell lysate, Hela cell lysate, Hela, NIH/3T3, HepG2. |
| Subcellular location: | Cytoplasm, Nucleus, Chromosome. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue |
1:1,000-1:5,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: Q9Y6K1 Human | O88508 Mouse | Q1LZ53 Rat |
| Alternative names: | DNA (cytosine 5) methyltransferase 3 alpha DNA (cytosine 5) methyltransferase 3A DNA (cytosine-5)-methyltransferase 3A DNA cytosine methyltransferase 3A2 DNA methyltransferase 3 alpha DNA methyltransferase 3a DNA methyltransferase HsaIIIA DNA MTase HsaIIIA DNM3A_HUMAN DNMT 3a DNMT Dnmt3a DNMT3A2 M.HsaIIIA MCMT OTTHUMP00000201149 TBRS |
Images
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Fig1:
Western blot analysis of Dnmt3a on different lysates with Rabbit anti-Dnmt3a antibody (ET1609-31) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 102 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-31) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of Dnmt3a in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-31, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of Dnmt3a in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-31, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: ICC staining of Dnmt3a in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-31, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.