Dnmt3a Recombinant Rabbit Monoclonal Antibody [ST04-78]
cat.: ET1609-31
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | ST04-78 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 102 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Dnmt3a aa 600-640. |
| Positive control: | C2C12 cell lysate, NIH/3T3 cell lysate, Mouse brain tissue lysate, Mouse skeletal muscle tissue lysate, SH-SY5Y cell lysate, Hela cell lysate, mouse testis tissue, rat testis tissue. |
| Subcellular location: | Cytoplasm, Nucleus, Chromosome. |
| Recommended Dilutions:
WB IF-Tissue IHC-P |
1:1,000-1:5,000 1:50-1:200 1:200-1:3,000 |
| Uniprot #: | SwissProt: Q9Y6K1 Human | O88508 Mouse | Q1LZ53 Rat |
| Alternative names: | DNA (cytosine 5) methyltransferase 3 alpha DNA (cytosine 5) methyltransferase 3A DNA (cytosine-5)-methyltransferase 3A DNA cytosine methyltransferase 3A2 DNA methyltransferase 3 alpha DNA methyltransferase 3a DNA methyltransferase HsaIIIA DNA MTase HsaIIIA DNM3A_HUMAN DNMT 3a DNMT Dnmt3a DNMT3A2 M.HsaIIIA MCMT OTTHUMP00000201149 TBRS |
Images
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Fig1:
Western blot analysis of Dnmt3a on different lysates with Rabbit anti-Dnmt3a antibody (ET1609-31) at 1/1,000 dilution. Lane 1: C2C12 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: Mouse brain tissue lysate Lane 4: Mouse skeletal muscle tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 102 kDa Observed band size: 130 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-31) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Dnmt3a on different lysates with Rabbit anti-Dnmt3a antibody (ET1609-31) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 102 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-31) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Western blot analysis of Dnmt3a on different lysates with Rabbit anti-Dnmt3a antibody (ET1609-31) at 1/2,000 dilution. Lane 1: HCT116-WT cell lysate Lane 2: HCT116-KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 102 kDa Observed band size: 130 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-31) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Dnmt3a antibody (ET1609-31) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-31) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-Dnmt3a antibody (ET1609-31) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-31) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.