DGCR8 Recombinant Rabbit Monoclonal Antibody [ST04-79]
cat.: ET1609-32
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IP |
| Clonality: | Monoclonal |
| Clone number: | ST04-79 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 86 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human DGCR8 aa 383-773 / 773. |
| Positive control: | PC12 cell lysate, NIH/3T3 cell lysate, Hela cell lysate, Hela, PC12. |
| Subcellular location: | Nucleus, cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IP |
1:1,000-1:2,000 1:50-1:200 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q8WYQ5 Human | Q9EQM6 Mouse Unigene: 17134 Rat |
| Alternative names: | DGCRK6 C22orf12 D16H22S788E D16Wis2 DGCR 8 Dgcr8 DGCR8 microprocessor complex subunit DGCR8_HUMAN DGCRK 6 DiGeorge syndrome critical region 8 DiGeorge syndrome critical region gene 8 Gy1 Microprocessor complex subunit DGCR8 pasha |
Images
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Fig1: ICC staining DGCR8 (1/200) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining DGCR8 (1/200) in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3:
Western blot analysis of DGCR8 on different lysates with Rabbit anti-DGCR8 antibody (ET1609-32) at 1/1,000 dilution. Lane 1: HepG2 (Human liver cancer cells) cell lysate Lane 2: Jurkat(Human T-lymphoblastic cells) cell lysate Lane 3: Mouse testis tissue lysate Lane 4: Rat testis tissue lysate Lysates/proteins at cell:20ug/lane,tissue:40ug/lane. Exposure time: 59s seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1609-32, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 86.0 kDa Observed band size: 83 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.