DGCR8 Recombinant Rabbit Monoclonal Antibody [ST04-79]
cat.: ET1609-32
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IP
Clonality: Monoclonal
Clone number: ST04-79
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 86 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human DGCR8 aa 383-773 / 773.
Positive control: PC12 cell lysate, NIH/3T3 cell lysate, Hela cell lysate, Hela, PC12.
Subcellular location: Nucleus, cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IP

1:1,000-1:2,000
1:50-1:200
1:50-1:200
Use at an assay dependent concentration.
Uniprot #: SwissProt: Q8WYQ5 Human | Q9EQM6 Mouse
Unigene: 17134 Rat
Alternative names: DGCRK6 C22orf12 D16H22S788E D16Wis2 DGCR 8 Dgcr8 DGCR8 microprocessor complex subunit DGCR8_HUMAN DGCRK 6 DiGeorge syndrome critical region 8 DiGeorge syndrome critical region gene 8 Gy1 Microprocessor complex subunit DGCR8 pasha
Images
ET1609-32_1.jpg Fig1: Western blot analysis of DGCR8 on different lysates using anti-DGCR8 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela cell lysate
Lane 2: PC12 cell lysate
Lane 3: NIH/3T3 cell lysate
ET1609-32_2.jpg Fig2: ICC staining DGCR8 (1/200) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1609-32_3.jpg Fig3: ICC staining DGCR8 (1/200) in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.