mGluR5 Recombinant Rabbit Monoclonal Antibody [ST51-00]
cat.: ET1609-36
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | ST51-00 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 132 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human mGluR5 aa 1,163-1,212 / 1,212. |
| Positive control: | Human brain tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, human brain tissue, mouse brain tissue, rat brain tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Tissue IHC-P IP |
1:1,000-1:2,000 1:50-1:200 1:200-1:1,000 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P41594 Human | Q3UVX5 Mouse | P31424 Rat |
| Alternative names: | Glutamate receptor metabotropic 5 GPRC1E Grm5 GRM5_HUMAN Metabotropic glutamate receptor 5 Metabotropic glutamate receptor 5 variant F Metabotropic glutamate receptor 5 variant G Metabotropic glutamate receptor 5 variant H mGlu5 mGluR5 PPP1R86 Protein phosphatase 1 regulatory subunit 86 |
Images
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Fig1:
Western blot analysis of mGluR5 on different lysates with Rabbit anti-mGluR5 antibody (ET1609-36) at 1/1,000 dilution. Lane 1: Human brain tissue lysate (RIPA lysis) Lane 2: Mouse brain tissue lysate (no heat) Lane 3: Mouse brain tissue lysate (hot lysis) Lane 4: Rat brain tissue lysate (no heat) Lysates/proteins at 20 µg/Lane. Predicted band size: 132 kDa Observed band size: 150 kDa Exposure time: Lane 1: 3 minutes; Lane 2-3: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-36) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-mGluR5 antibody (ET1609-36) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-36) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-mGluR5 antibody (ET1609-36) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-36) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-mGluR5 antibody (ET1609-36) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-36) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.