Metabotropic glutamate receptor 5 Recombinant Rabbit Monoclonal Antibody [ST51-00]
cat.: ET1609-36
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | ST51-00 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 132 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human GRM5 aa 1,163-1,212 / 1,212. |
| Positive control: | PC-12 cell lysates, JAR, PC-12, SHG-44, rat brain tissue, mouse brain tissue, SH-SY5Y. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC IP |
1:1,000-1:2,000 1:50-1:200 1:50-1:200 1:200-1:500 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P41594 Human | Q3UVX5 Mouse | P31424 Rat |
| Alternative names: | Glutamate receptor metabotropic 5 GPRC1E Grm5 GRM5_HUMAN Metabotropic glutamate receptor 5 Metabotropic glutamate receptor 5 variant F Metabotropic glutamate receptor 5 variant G Metabotropic glutamate receptor 5 variant H mGlu5 mGluR5 PPP1R86 Protein phosphatase 1 regulatory subunit 86 |
Images
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Fig1: Western blot analysis of Metabotropic glutamate receptor 5 on PC-12 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-36, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of Metabotropic glutamate receptor 5 in JAR cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-36, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of Metabotropic glutamate receptor 5 in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-36, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: ICC staining of Metabotropic glutamate receptor 5 in SHG-44 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-36, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Metabotropic glutamate receptor 5 antibody (ET1609-36) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-36) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Metabotropic glutamate receptor 5 antibody (ET1609-36) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-36) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7: Flow cytometric analysis of Metabotropic glutamate receptor 5 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1609-36, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.