PRMT5 Recombinant Rabbit Monoclonal Antibody [ST51-06]
cat.: ET1609-43
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | ST51-06 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 73 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PRMT5 aa 48-91 / 637. |
| Positive control: | HepG2 cell lysate, HEK-293 cell lysate, Raji cell lysate, Jurkat cell lysate, A431 cell lysate, C6 cell lysate, PC-12 cell lysate, bEnd.3 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, HepG2, MCF7 cell lysate, COS-1 cell lysate, Neuro-2a cell lysate, Neuro-2a, human kidney tissue, mouse ovary tissue, rat ovary tissue. |
| Subcellular location: | Cytoplasm, Nucleus, Golgi apparatus, Chromosome. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P |
1:500-1:5,000 1:50-1:250 1:50-1:200 1:500-1:2,000 |
| Uniprot #: | SwissProt: O14744 Human | Q8CIG8 Mouse | D4A0E8 Rat |
| Alternative names: | 72 kDa ICln binding protein 72 kDa ICln-binding protein ANM5_HUMAN Histone synthetic lethal 7, S. cerevisiae, homolog of Histone-arginine N-methyltransferase PRMT5 HMT1 hnRNP methyltransferase like 5 HOMOLOG OF; SKB1 HRMT1L5 IBP72 Jak-binding protein 1 JBP 1 JBP1 PRMT 5 PRMT5 Protein arginine methyltransferase 5 Protein arginine N methyltransferase 5 Protein arginine N methyltransferase 5 N terminally processed Protein arginine N-methyltransferase 5 S. POMBE S. POMBE HOMOLOG OF; SKB1 SHK1 KINASE BINDING PROTEIN 1 Shk1 kinase binding protein 1 homolog Shk1 kinase-binding protein 1 homolog Shk1 kinase/binding protein 1, S. pombe, homolog of SKB 1 SKB1 SKB1 homolog SKB1: SKB1 homolog (S. pombe) SKB1Hs |
Images
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Fig1:
Western blot analysis of PRMT5 on different lysates with Rabbit anti-PRMT5 antibody (ET1609-43) at 1/5,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HEK-293 cell lysate Lane 3: Raji cell lysate Lane 4: Jurkat cell lysate Lane 5: A431 cell lysate Lane 6: C6 cell lysate Lane 7: PC-12 cell lysate Lane 8: bEnd.3 cell lysate Lane 9: NIH/3T3 cell lysate Lane 10: C2C12 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 73 kDa Observed band size: 70 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-43) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HepG2 cells labeling PRMT5 with Rabbit anti-PRMT5 antibody (ET1609-43) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PRMT5 antibody (ET1609-43) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Western blot analysis of PRMT5 on different lysates with Rabbit anti-PRMT5 antibody (ET1609-43) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HEK-293 cell lysate Lane 3: Raji cell lysate Lane 4: Jurkat cell lysate Lane 5: MCF7 cell lysate Lane 6: A431 cell lysate Lane 7: COS-1 cell lysate Lane 8: Neuro-2a cell lysate Lane 9: bEnd.3 cell lysate Lane 10: NIH/3T3 cell lysate Lane 11: C2C12 cell lysate Lane 12: C6 cell lysate Lane 13: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 73 kDa Observed band size: 70 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-43) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Immunocytochemistry analysis of Neuro-2a cells labeling PRMT5 with Rabbit anti-PRMT5 antibody (ET1609-43) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PRMT5 antibody (ET1609-43) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PRMT5 antibody (ET1609-43) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-43) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue with Rabbit anti-PRMT5 antibody (ET1609-43) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-43) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded rat ovary tissue with Rabbit anti-PRMT5 antibody (ET1609-43) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-43) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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