PRMT5 Recombinant Rabbit Monoclonal Antibody [ST51-06]
cat.: ET1609-43
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC-P
Clonality: Monoclonal
Clone number: ST51-06
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 73 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human PRMT5 aa 48-91 / 637.
Positive control: HL-60 cell lysates, A431 cell lysates, HepG2, human breast carcinoma tissue, human kidney tissue, mouse liver tissue, mouse kidney tissue.
Subcellular location: Cytoplasm, Nucleus, Golgi apparatus, Chromosome.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P

1:500-1:2,000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: O14744 Human | Q8CIG8 Mouse | D4A0E8 Rat
Alternative names: 72 kDa ICln binding protein 72 kDa ICln-binding protein ANM5_HUMAN Histone synthetic lethal 7, S. cerevisiae, homolog of Histone-arginine N-methyltransferase PRMT5 HMT1 hnRNP methyltransferase like 5 HOMOLOG OF; SKB1 HRMT1L5 IBP72 Jak-binding protein 1 JBP 1 JBP1 PRMT 5 PRMT5 Protein arginine methyltransferase 5 Protein arginine N methyltransferase 5 Protein arginine N methyltransferase 5 N terminally processed Protein arginine N-methyltransferase 5 S. POMBE S. POMBE HOMOLOG OF; SKB1 SHK1 KINASE BINDING PROTEIN 1 Shk1 kinase binding protein 1 homolog Shk1 kinase-binding protein 1 homolog Shk1 kinase/binding protein 1, S. pombe, homolog of SKB 1 SKB1 SKB1 homolog SKB1: SKB1 homolog (S. pombe) SKB1Hs
Images
ET1609-43_1.jpg Fig1: Western blot analysis of PRMT5 on different cell lysates.
Lane1: HL-60 cell lysates
Lane2: A431 cell lysates

Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-43, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1609-43_2.jpg Fig2: ICC staining of PRMT5 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-43, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1609-43_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PRMT5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-43, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1609-43_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PRMT5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-43, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1609-43_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-PRMT5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-43, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1609-43_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PRMT5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-43, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.