beta Ⅱ Tubulin Recombinant Rabbit Monoclonal Antibody [ST52-04]
cat.: ET1609-48
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Human |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | ST52-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human beta Ⅱ Tubulin aa 407-445 / 445. |
| Positive control: | Mouse brain tissue lysate, mouse kidney tissue lysate, N2A, mouse kidney tissue, mouse brain tissue, rat brain tissue. |
| Subcellular location: | Cytoplasm, Cytoskeleton, Microtubule. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P IP |
1:1,000-1:5,000 1:50-1:200 1:50-1:200 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P68371 Human | Q13885 Human | Q9BVA1 Human | P68372 Mouse | Q7TMM9 Mouse | Q9CWF2 Mouse | P85108 Rat | Q3KRE8 Rat | Q6P9T8 Rat |
| Alternative names: | bA506K6.1 Beta2 class II beta tubulin isotype class IIa beta-tubulin class IIb beta-tubulin Class IVb beta tubulin dJ40E16.7 DKFZp566F223 FLJ98847 M(beta)2 MGC8685 OTTHUMP00000015956 OTTHUMP00000015964 TBB4B_HUMAN TUBB 2 TUBB 2A TUBB 2C TUBB TUBB PARALOG TUBB2 TUBB2A TUBB2B TUBB2C Tubb4b Tubulin beta 2 Tubulin beta 2 chain Tubulin beta 2A Tubulin beta 2A chain Tubulin beta 2B Tubulin beta 2B chain Tubulin beta 2C Tubulin beta polypeptide Tubulin beta polypeptide 2 Tubulin beta polypeptide paralog Tubulin beta-2 chain Tubulin beta-2C chain Tubulin beta-4B chain tubulin, beta 2A class IIa tubulin, beta 2B class IIb tubulin, beta 4B class IVb Tubulin, beta, class IVB Tubulin, beta-4B |
Images
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Fig1:
Western blot analysis of beta Ⅱ Tubulin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-48, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse brain tissue lysate Lane 2: mouse kidney tissue lysate |
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Fig2: ICC staining of beta Ⅱ Tubulin in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-48, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-beta Ⅱ Tubulin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-48, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-beta Ⅱ Tubulin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-48, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-beta Ⅱ Tubulin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-48, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.