beta II Tubulin Recombinant Rabbit Monoclonal Antibody [ST52-04]
cat.: ET1609-48
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Human |
| Applications: | WB, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | ST52-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human beta Ⅱ Tubulin aa 407-445 / 445. |
| Positive control: | C2C12 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, mouse kidney tissue, mouse brain tissue, rat brain tissue. |
| Subcellular location: | Cytoplasm, Cytoskeleton, Microtubule. |
| Recommended Dilutions:
WB IF-Tissue IHC-P IP |
1:1,000-1:5,000 1:50-1:200 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P68371 Human | Q13885 Human | Q9BVA1 Human | P68372 Mouse | Q7TMM9 Mouse | Q9CWF2 Mouse | P85108 Rat | Q3KRE8 Rat | Q6P9T8 Rat |
| Alternative names: | bA506K6.1 Beta2 class II beta tubulin isotype class IIa beta-tubulin class IIb beta-tubulin Class IVb beta tubulin dJ40E16.7 DKFZp566F223 FLJ98847 M(beta)2 MGC8685 OTTHUMP00000015956 OTTHUMP00000015964 TBB4B_HUMAN TUBB 2 TUBB 2A TUBB 2C TUBB TUBB PARALOG TUBB2 TUBB2A TUBB2B TUBB2C Tubb4b Tubulin beta 2 Tubulin beta 2 chain Tubulin beta 2A Tubulin beta 2A chain Tubulin beta 2B Tubulin beta 2B chain Tubulin beta 2C Tubulin beta polypeptide Tubulin beta polypeptide 2 Tubulin beta polypeptide paralog Tubulin beta-2 chain Tubulin beta-2C chain Tubulin beta-4B chain tubulin, beta 2A class IIa tubulin, beta 2B class IIb tubulin, beta 4B class IVb Tubulin, beta, class IVB Tubulin, beta-4B beta Ⅱ Tubulin beta Ⅱ Tubulin |
Images
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Fig1:
Western blot analysis of beta II Tubulin on different lysates with Rabbit anti-beta II Tubulin antibody (ET1609-48) at 1/1,000 dilution. Lane 1: C2C12 cell lysate (20 µg/Lane) Lane 2: Mouse brain tissue lysate (40 µg/Lane) Lane 3: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: Lane 1: 30 seconds; Lane 2-3: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-48) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-beta II Tubulin antibody (ET1609-48) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-48) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-beta II Tubulin antibody (ET1609-48) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-48) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-beta II Tubulin antibody (ET1609-48) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-48) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.