beta II Tubulin Recombinant Rabbit Monoclonal Antibody [ST52-04]
cat.: ET1609-48
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Mouse, Rat, Human
Applications: WB, IF-Tissue, IHC-P
Clonality: Monoclonal
Clone number: ST52-04
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 50 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human beta Ⅱ Tubulin aa 407-445 / 445.
Positive control: C2C12 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, mouse kidney tissue, mouse brain tissue, rat brain tissue.
Subcellular location: Cytoplasm, Cytoskeleton, Microtubule.
Recommended Dilutions:
  WB
  IF-Tissue
  IHC-P

1:1,000-1:5,000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: P68371 Human | Q13885 Human | Q9BVA1 Human | P68372 Mouse | Q7TMM9 Mouse | Q9CWF2 Mouse | P85108 Rat | Q3KRE8 Rat | Q6P9T8 Rat
Alternative names: bA506K6.1 Beta2 class II beta tubulin isotype class IIa beta-tubulin class IIb beta-tubulin Class IVb beta tubulin dJ40E16.7 DKFZp566F223 FLJ98847 M(beta)2 MGC8685 OTTHUMP00000015956 OTTHUMP00000015964 TBB4B_HUMAN TUBB 2 TUBB 2A TUBB 2C TUBB TUBB PARALOG TUBB2 TUBB2A TUBB2B TUBB2C Tubb4b Tubulin beta 2 Tubulin beta 2 chain Tubulin beta 2A Tubulin beta 2A chain Tubulin beta 2B Tubulin beta 2B chain Tubulin beta 2C Tubulin beta polypeptide Tubulin beta polypeptide 2 Tubulin beta polypeptide paralog Tubulin beta-2 chain Tubulin beta-2C chain Tubulin beta-4B chain tubulin, beta 2A class IIa tubulin, beta 2B class IIb tubulin, beta 4B class IVb Tubulin, beta, class IVB Tubulin, beta-4B beta Ⅱ Tubulin beta Ⅱ Tubulin
Images
ET1609-48_1.jpg Fig1: Western blot analysis of beta II Tubulin on different lysates with Rabbit anti-beta II Tubulin antibody (ET1609-48) at 1/1,000 dilution.

Lane 1: C2C12 cell lysate (20 µg/Lane)
Lane 2: Mouse brain tissue lysate (40 µg/Lane)
Lane 3: Rat brain tissue lysate (40 µg/Lane)

Predicted band size: 50 kDa
Observed band size: 50 kDa

Exposure time: Lane 1: 30 seconds; Lane 2-3: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-48) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1609-48_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-beta II Tubulin antibody (ET1609-48) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-48) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1609-48_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-beta II Tubulin antibody (ET1609-48) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-48) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1609-48_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-beta II Tubulin antibody (ET1609-48) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-48) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.