ROCK1 Recombinant Rabbit Monoclonal Antibody [ST05-19]
cat.: ET1609-53
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | ST05-19 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 158 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ROCK1 aa aa 1104-1149 / 1,354. |
| Positive control: | A431 cell lysate, NIH/3T3 cell lysate, mouse thyroid tissue, mouse heart tissue, human thyroid tissue. |
| Subcellular location: | Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P IP |
1:500-1:5,000 1:50-1:200 1:50-1:200 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q13464 Human | P70335 Mouse | Q63644 Rat |
| Alternative names: | coiled-coil-containing protein kinase 1 coiled-coil-containing protein kinase I MGC131603 MGC43611 p160 Rhoassociated coiled coil-forming protein kinase p160 ROCK-1 p160 ROCK1 p160ROCK PRO0435 Renal carcinoma antigen NY REN 35 Renal carcinoma antigen NY-REN-35 Rho associated coiled coil containing protein kinase 1 Rho associated protein kinase 1 Rho kinase Rho-alpha kinase Rho-associated Rho-associated protein kinase 1 ROCK I ROCK-I ROCK1 ROCK1_HUMAN Rok rokalpha |
Images
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Fig1:
Western blot analysis of ROCK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-53, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A431 cell lysate Lane 2: NIH/3T3 cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue using anti-ROCK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-53, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ROCK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-53, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-ROCK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-53, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.