Phospho-BTK (Y223) Recombinant Rabbit Monoclonal Antibody [ST51-05]
cat.: ET1609-55
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | ST51-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 76 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Tyr223 of human BTK. |
| Positive control: | Ramos cell lysate, Ramos treated with 12μg/mL human IgM for 10 minutes cell lysate, K562 cells treated with pervanadate. |
| Subcellular location: | Cytoplasm, Nucleus, Cell membrane. |
| Recommended Dilutions:
WB IP |
1:1,000-1:2,000 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q06187 Human | P35991 Mouse |
| Alternative names: | Agammaglobulinaemia tyrosine kinase AGMX 1 AGMX1 AT ATK B cell progenitor kinase B-cell progenitor kinase BPK Bruton agammaglobulinemia tyrosine kinase Bruton tyrosine kinase Bruton’s Tyrosine Kinase Btk BTK_HUMAN dominant-negative kinase-deficient Brutons tyrosine kinase IMD 1 IMD1 MGC126261 MGC126262 OTTHUMP00000063593 PSCTK 1 PSCTK1 truncated Bruton agammaglobulinemia tyrosine kinase Tyrosine protein kinase BTK Tyrosine-protein kinase BTK tyrosine-protein kinase BTK isoform (lacking exon 14 XLA |
Images
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Fig1:
Western blot analysis of Phospho-BTK (Y223) on different lysates with Rabbit anti-Phospho-BTK (Y223) antibody (ET1609-55) at 1/1,000 dilution. Lane 1: Ramos cell lysate Lane 2: Ramos treated with 12μg/mL human IgM for 10 minutes cell lysate Lane 3: Ramos treated with 12μg/mL human IgM for 10 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 76 kDa Observed band size: 76 kDa Exposure time: 59 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-55) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Phospho-BTK(Y223) on different lysates using anti-Phospho-BTK(Y223) antibody at 1/1,000 dilution. Positive control: Lane 1: K562 cells treated with pervanadate Lane 2: Untreated K562 cell lysate |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.