IgG4 Recombinant Rabbit Monoclonal Antibody [ST05-12]
cat.: ET1609-56
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | ST05-12 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human IgG4 aa 85-129 / 327. |
| Positive control: | Human serum lysate, Human plasma lysates, human tonsil tissue, human spleen tissue. |
| Subcellular location: | Cell membrane, Immunoglobulin, Membrane, Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:5,000 1:50-1:200 |
| Uniprot #: | SwissProt: P01861 Human |
| Alternative names: | Ig gamma 4 chain C region IGHG4 Immunoglobulin heavy constant gamma 4 (G4m marker) MGC117419 |
Images
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Fig1:
Western blot analysis of IgG4 on different lysates with Rabbit anti-IgG4 antibody (ET1609-56) at 1/2,000 dilution. Lane 1: Human serum tissue lysate Lane 2: Human plasma tissue lysate Lysates/proteins at 40 µg/Lane. Exposure time: 2 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1609-56, 1/5,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 43.8 kDa Observed band size: 55 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IgG4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-56, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IgG4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-56, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.