Anti-Cyclophilin B antibody [ST05-22]
cat.: ET1609-58
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: ST05-22
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 18 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human Cyclophilin B aa 180-216.
Positive control: Hela cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, rat epididymis tissue, human thyroid tissue, human placenta tissue.
Subcellular location: Endoplasmic reticulum lumen, Melanosome.
Recommended Dilutions:
  WB
  IHC-P

1:500-1:5,000
1:50-1:200
Uniprot #: SwissProt: P23284 Human | P24369 Mouse | P24368 Rat
Alternative names: AA408962 antibody AA553318 antibody AI844835 antibody Cphn 2 antibody Cphn2 antibody Cyclophilin B antibody Cyclophilin like protein antibody CyP 20b antibody CYP S1 antibody CYP-S1 antibody CYPB antibody EC 5.2.1.8 antibody MGC14109 antibody MGC2224 antibody OI9 antibody peptidyl prolyl cis trans isomerase B antibody Peptidyl prolyl cis trans isomerase B precursor antibody Peptidyl-prolyl cis-trans isomerase B antibody peptidylprolyl isomerase B (cyclophilin B) antibody Peptidylprolyl isomerase B antibody PPIase antibody PPIase B antibody Ppib antibody PPIB_HUMAN antibody Rotamase antibody Rotamase B antibody S cyclophilin antibody S-cyclophilin antibody SCYLP antibody
Images
ET1609-58_1.jpg Fig1: Western blot analysis of Cyclophilin B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-58, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: NIH/3T3 cell lysate
ET1609-58_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Cyclophilin B antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-58, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1609-58_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-Cyclophilin B antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-58, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1609-58_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Cyclophilin B antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-58, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.