Cyclophilin B Recombinant Rabbit Monoclonal Antibody [ST05-22]
cat.: ET1609-58
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | ST05-22 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 24 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Cyclophilin B aa 171-215 / 216. |
| Positive control: | HepG2 cell lysate, HeLa cell lysate, U-87 MG cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, rat epididymis tissue, human thyroid tissue, human placenta tissue. |
| Subcellular location: | Virion, Endoplasmic reticulum lumen, Melanosome. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:5,000 1:50-1:200 |
| Uniprot #: | SwissProt: P23284 Human | P24369 Mouse | P24368 Rat |
| Alternative names: | AA408962 AA553318 AI844835 Cphn 2 Cphn2 Cyclophilin B Cyclophilin like protein CyP 20b CYP S1 CYP-S1 CYPB EC 5.2.1.8 MGC14109 MGC2224 OI9 peptidyl prolyl cis trans isomerase B Peptidyl prolyl cis trans isomerase B precursor Peptidyl-prolyl cis-trans isomerase B peptidylprolyl isomerase B (cyclophilin B) Peptidylprolyl isomerase B PPIase PPIase B Ppib PPIB_HUMAN Rotamase Rotamase B S cyclophilin S-cyclophilin SCYLP |
Images
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Fig1:
Western blot analysis of Cyclophilin B on different lysates with Rabbit anti-Cyclophilin B antibody (ET1609-58) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: U-87 MG cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 24 kDa Observed band size: 20 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-58) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Cyclophilin B antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-58, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-Cyclophilin B antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-58, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Cyclophilin B antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-58, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.