nNOS Recombinant Rabbit Monoclonal Antibody [ST520]
cat.: ET1609-61
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | ST520 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 161 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human nNOS aa 1398-1434 / 1434. |
| Positive control: | Mouse brain tissue lysate, Mouse hippocampus tissue lysate, Mouse cerebellum tissue lysate, Rat hippocampus tissue lysate, Rat cerebellum tissue lysate, human skeletal muscle tissue, rat brain tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue. |
| Subcellular location: | Cell membrane, sarcolemma, Cell projection, dendritic spine. |
| Recommended Dilutions:
WB IF-Tissue IHC-P IP |
1:1,000-1:2,000 1:50-1:200 1:200-1:1,000 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P29475 Human | Q9Z0J4 Mouse | P29476 Rat |
| Alternative names: | 2310005C01Rik BNOS Constitutive NOS EC 1.14.13.39 IHPS 1 IHPS1 N-NOS NC-NOS neuronal Nitric Oxide Synthase Neuronal NOS Nitric oxide synthase , neuronal, included Nitric oxide synthase 1 (neuronal) Nitric oxide synthase 1 Nitric oxide synthase, brain Nitric oxide synthase, penile neuronal, included NNOS NO NOS 1 NOS NOS type I NOS-I NOS1 NOS1_HUMAN Peptidyl-cysteine S-nitrosylase NOS1 |
Images
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Fig1:
Western blot analysis of nNOS on different lysates with Rabbit anti-nNOS antibody (ET1609-61) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate (40 µg/Lane) Lane 2: Mouse hippocampus tissue lysate (40 µg/Lane) Lane 3: Mouse cerebellum tissue lysate (40 µg/Lane) Lane 4: Rat hippocampus tissue lysate (40 µg/Lane) Lane 5: Rat cerebellum tissue lysate (40 µg/Lane) Predicted band size: 161 kDa Observed band size: 161 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-61) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-nNOS antibody (ET1609-61) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-61) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-nNOS antibody (ET1609-61) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-61) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-nNOS antibody (ET1609-61) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-61) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-nNOS antibody (ET1609-61) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-61) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-nNOS antibody (ET1609-61) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-61) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.