PGK1 Recombinant Rabbit Monoclonal Antibody [ST49-07]
cat.: ET1609-63
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | ST49-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PGK1 aa 1-49 / 417. |
| Positive control: | HT-29 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, PC-12 cell lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000-1:5,000 |
| Uniprot #: | SwissProt: P00558 Human | P09411 Mouse | P16617 Rat |
| Alternative names: | Cell migration-inducing gene 10 protein Epididymis secretory sperm binding protein Li 68p HEL S 68p MGC117307 MGC8947 MIG10 pgk1 PGK1_HUMAN PGKA Phosphoglycerate kinase 1 Primer recognition protein 2 PRP 2 |
Images
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Fig1:
Western blot analysis of PGK1 on different lysates with Rabbit anti-PGK1 antibody (ET1609-63) at 1/2,000 dilution. Lane 1: HT-29 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: C2C12 cell lysate Lane 4: C6 cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 9 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-63) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.