Anti-PGK1 antibody [ST49-07]
cat.: ET1609-63
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IP, FC
Clonality: Monoclonal
Clone number: ST49-07
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 45 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal human PGK1.
Positive control: Hela cell lysate, MCF-7 cell lysate, HepG2 cell lysate, HepG2, Hela.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  ICC/IF
  FC

1:1,000-1:5,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P00558 Human | P09411 Mouse | P16617 Rat
Alternative names: Cell migration-inducing gene 10 protein antibody Epididymis secretory sperm binding protein Li 68p antibody HEL S 68p antibody MGC117307 antibody MGC8947 antibody MIG10 antibody pgk1 antibody PGK1_HUMAN antibody PGKA antibody Phosphoglycerate kinase 1 antibody Primer recognition protein 2 antibody PRP 2 antibody
Images
ET1609-63_1.jpg Fig1: Western blot analysis of PGK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-63, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: HepG2 cell lysate
ET1609-63_2.jpg Fig2: ICC staining of PGK1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-63, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1609-63_3.jpg Fig3: Flow cytometric analysis of PGK1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1609-63, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.