Phospho-c-Myc (S62) Recombinant Rabbit Monoclonal Antibody [ST49-08]
cat.: ET1609-64
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, IP
Clonality: Monoclonal
Clone number: ST49-08
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 57 kDa
Isotype: IgG
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser62 of Human c-Myc aa 46-87 / 439.
Positive control: A549 cell lysate, HCT116 cell lysate, Hela cell lysate, HepG2 cell lysate, Hela.
Subcellular location: Nucleoplasm, nucleolus.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  IF-Tissue
  IP

1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:200
Use at an assay dependent concentration.
Uniprot #: SwissProt: P01106 Human | P01108 Mouse | P09416 Rat
Alternative names: AU016757 Avian myelocytomatosis viral oncogene homolog bHLHe39 c Myc Class E basic helix-loop-helix protein 39 MRTL Myc Myc protein Myc proto oncogene protein Myc proto-oncogene protein myc-related translation/localization regulatory factor MYC_HUMAN Myc2 MYCC Myelocytomatosis oncogene Niard Nird Oncogene Myc OTTHUMP00000158589 Proto-oncogene c-Myc Protooncogene homologous to myelocytomatosis virus RNCMYC Transcription factor p64 Transcriptional regulator Myc-A V-Myc avian myelocytomatosis viral oncogene homolog v-myc myelocytomatosis viral oncogene homolog (avian)
Images
ET1609-64_1.jpg Fig1: Western blot analysis of Phospho-c-Myc (S62) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-64, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A549 cell lysate
Lane 2: HCT116 cell lysate
Lane 3: Hela cell lysate
Lane 4: HepG2 cell lysate
ET1609-64_2.jpg Fig2: Western blot analysis of Phospho-c-Myc(S62) on HepG2 cell lysates.

Lane 1: HepG2 cells, whole cell lysate, 10ug/lane
Lane 2: HepG2 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane

All lanes :
Anti-Phospho-c-Myc(S62) antibody (ET1609-64) at 1:500 dilution. Anti-GAPDH antibody (ET1601-4) at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution.

Predicted band size: 49 kDa
Observed band size: 57 kDa

Blocking and diluting buffer: 5% BSA.

Exposure time: 3 minutes
ET1609-64_3.jpg Fig3: Western blot analysis of Phospho-c-Myc(S62) on Hela cell lysates.

Lane 1: Hela cells, whole cell lysate, 10ug/lane
Lane 2/3: Hela cells treated with 200 nM PMA for 10 minutes, whole cell lysates, 10ug/lane
Lane 4: Hela cells treated with 200 nM PMA for 10 minutes, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane

All lanes :
Anti-Phospho-c-Myc(S62) antibody (ET1609-64
) at 1:500 dilution. Anti-GAPDH antibody (ET1601-4) at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution.

Predicted band size: 57 kDa
Observed band size:60 kDa

Blocking and diluting buffer: 5% BSA.

Exposure time: 3 minutes
ET1609-64_4.jpg Fig4: ICC staining of Phospho-c-Myc(S62) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-64, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.