Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP |
Clonality: | Monoclonal |
Clone number: | ST49-08 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 57 kDa |
Isotype: | IgG |
Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Ser62 of Human c-Myc aa 46-87 / 439. |
Positive control: | A549 cell lysate, HCT116 cell lysate, Hela cell lysate, HepG2 cell lysate, Hela. |
Subcellular location: | Nucleoplasm, nucleolus. |
Recommended Dilutions:
WB IHC-P IF-Cell IF-Tissue IP |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:200 Use at an assay dependent concentration. |
Uniprot #: | SwissProt: P01106 Human | P01108 Mouse | P09416 Rat |
Alternative names: | AU016757 Avian myelocytomatosis viral oncogene homolog bHLHe39 c Myc Class E basic helix-loop-helix protein 39 MRTL Myc Myc protein Myc proto oncogene protein Myc proto-oncogene protein myc-related translation/localization regulatory factor MYC_HUMAN Myc2 MYCC Myelocytomatosis oncogene Niard Nird Oncogene Myc OTTHUMP00000158589 Proto-oncogene c-Myc Protooncogene homologous to myelocytomatosis virus RNCMYC Transcription factor p64 Transcriptional regulator Myc-A V-Myc avian myelocytomatosis viral oncogene homolog v-myc myelocytomatosis viral oncogene homolog (avian) |
Fig1:
Western blot analysis of Phospho-c-Myc (S62) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-64, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: HCT116 cell lysate Lane 3: Hela cell lysate Lane 4: HepG2 cell lysate |
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Fig2:
Western blot analysis of Phospho-c-Myc(S62) on HepG2 cell lysates. Lane 1: HepG2 cells, whole cell lysate, 10ug/lane Lane 2: HepG2 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-c-Myc(S62) antibody (ET1609-64) at 1:500 dilution. Anti-GAPDH antibody (ET1601-4) at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution. Predicted band size: 49 kDa Observed band size: 57 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 3 minutes |
Fig3:
Western blot analysis of Phospho-c-Myc(S62) on Hela cell lysates. Lane 1: Hela cells, whole cell lysate, 10ug/lane Lane 2/3: Hela cells treated with 200 nM PMA for 10 minutes, whole cell lysates, 10ug/lane Lane 4: Hela cells treated with 200 nM PMA for 10 minutes, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-c-Myc(S62) antibody (ET1609-64 ) at 1:500 dilution. Anti-GAPDH antibody (ET1601-4) at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution. Predicted band size: 57 kDa Observed band size:60 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 3 minutes |
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Fig4: ICC staining of Phospho-c-Myc(S62) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-64, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |