Src Recombinant Rabbit Monoclonal Antibody [ST05-03]
cat.: ET1609-65
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | ST05-03 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 60 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Src aa 437-536 / 536. |
| Positive control: | A549 cell lysate, A431 cell lysate, mouse cerebellum tissue lysate, mouse lung tissue lysate, mouse liver tissue lysate, rat cerebellum tissue lysate, rat lung tissue lysate, Hela, rat testis tissue. |
| Subcellular location: | Cell membrane, Mitochondrion inner membrane, Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P |
1:1,000 1:50-1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P12931 Human | P05480 Mouse | Q9WUD9 Rat |
| Alternative names: | ASV Avian sarcoma virus c SRC CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA cSrc EC 2.7.10.2 Neuronal CSRC tyrosine specific protein kinase Neuronal SRC Oncogene SRC OTTHUMP00000174476 OTTHUMP00000174477 p60 Src p60-Src p60Src pp60c src pp60c-src pp60csrc Proto oncogene tyrosine protein kinase Src Proto-oncogene c-Src Proto-oncogene tyrosine-protein kinase Src Protooncogene SRC Protooncogene SRC Rous sarcoma Src SRC Oncogene SRC proto oncogene non receptor tyrosine kinase SRC_HUMAN SRC1 Tyrosine kinase pp60c src Tyrosine protein kinase SRC 1 Tyrosine protein kinase SRC1 v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian) v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian |
Images
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Fig1:
Western blot analysis of Src on different lysates with Rabbit anti-Src antibody (ET1609-65) at 1/1,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD Src cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 56 kDa Observed band size: 54 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-65) at 1/1,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Src on different lysates with Rabbit anti-Src antibody (ET1609-65) at 1/1,000 dilution. Lane 1: A549 cell lysate (10 µg/Lane) Lane 2: A431 cell lysate (10 µg/Lane) Lane 3: Mouse cerebellum tissue lysate (20 µg/Lane) Lane 4: Mouse lung tissue lysate (20 µg/Lane) Lane 5: Mouse liver tissue lysate (20 µg/Lane) Lane 6: Rat cerebellum tissue lysate (20 µg/Lane) Lane 7: Rat lung tissue lysate (20 µg/Lane) Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-65) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3: ICC staining Src in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Src antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.