HIF-1 beta Recombinant Rabbit Monoclonal Antibody [ST05-25]
cat.: ET1609-67
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | ST05-25 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 87 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human HIF-1 beta aa 479-789 / 789. |
| Positive control: | Human liver tissue lysates, human liver tissue, human fallopian tube tissue, human placenta tissue, human uterus tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: P27540 Human |
| Alternative names: | arnT ARNT protein ARNT_HUMAN Aryl hydrocarbon receptor nuclear translocator bHLHe2 Class E basic helix-loop-helix protein 2 Dioxin receptor Dioxin receptor nuclear translocator Drnt HIF 1 beta HIF 1beta HIF-1-beta HIF1-beta HIF1B HIF1beta Hypoxia Inducible Factor 1 Hypoxia inducible factor 1 beta Hypoxia-inducible factor 1-beta nuclear translocator Tango |
Images
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Fig1:
Western blot analysis of HIF-1 beta on different lysates with Rabbit anti-HIF-1 beta antibody (ET1609-67) at 1/5,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si HIF-1 beta cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 87 kDa Observed band size: 72/87 kDa Exposure time: 1 minute 20 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-67) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis of HIF-1 beta on human liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-67, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-HIF-1 beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-67, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue using anti-HIF-1 beta antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-67, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-HIF-1 beta antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-67, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-HIF-1 beta antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-67, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.