Anti-COL1A1/Collagen Ⅰ antibody [ST58-04]
cat.: ET1609-68
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Cow
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: ST58-04
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 130 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human COL1A1 aa 1200-1240.
Positive control: Human placenta tissue lysates, human kidney tissue, human uterus tissue.
Subcellular location: Secreted.
Recommended Dilutions:
  WB
  IHC-P

1:1,000-1:2,000
1:50-1:200
Uniprot #: SwissProt: P02452 Human | P08123 Human
Alternative names: Alpha 1 type I collagen antibody Alpha 2 type I collagen antibody alpha 2 type I procollagen antibody alpha 2(I) procollagen antibody alpha 2(I)-collagen antibody Alpha-1 type I collagen antibody alpha1(I) procollagen antibody CO1A1_HUMAN antibody COL1A1 antibody COL1A2 antibody collagen alpha 1 chain type I antibody Collagen alpha-1(I) chain antibody collagen alpha-1(I) chain preproprotein antibody Collagen I alpha 1 polypeptide antibody Collagen I alpha 2 polypeptide antibody collagen of skin, tendon and bone, alpha-1 chain antibody collagen of skin, tendon and bone, alpha-2 chain antibody Collagen type I alpha 1 antibody Collagen type I alpha 2 antibody EDSC antibody OI1 antibody OI2 antibody OI3 antibody OI4 antibody pro-alpha-1 collagen type 1 antibody type I proalpha 1 antibody type I procollagen alpha 1 chain antibody Type I procollagen antibody
Images
ET1609-68_1.jpg Fig1: Western blot analysis of COL1A1/Collagen Ⅰ on human placenta tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-68, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1609-68_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-COL1A1/Collagen Ⅰ antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-68, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1609-68_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-COL1A1/Collagen Ⅰ antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-68, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.