HtrA2 / Omi Recombinant Rabbit Monoclonal Antibody [ST54-05]
cat.: ET1609-73
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | ST54-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 49 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human HtrA2 aa 80-188 / 458. |
| Positive control: | HEK-293 cell lysate, Jurkat cell lysate, HeLa cell lysate, SH-SY5Y cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, human liver tissue, rat liver tissue, mouse liver tissue. |
| Subcellular location: | Mitochondrion intermembrane space, Mitochondrion membrane. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: O43464 Human | Q9JIY5 Mouse Unigene: 107325 Rat |
| Alternative names: | High temperature requirement protein A2 HTRA 2 HtrA like serine protease HtrA serine peptidase 2 HtrA, E. coli, homolog of, 2 HtrA2 HTRA2_HUMAN mitochondrial Omi stress regulated endoprotease Omi stress-regulated endoprotease PARK 13 PARK13 Protease serine 25 PRSS 25 PRSS25 Serine protease 25 Serine protease HTRA2 Serine protease HTRA2 mitochondrial Serine protease htra2 mitochondrial precursor Serine protease omi Serine proteinase OMI |
Images
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Fig1:
Western blot analysis of HtrA2 / Omi on different lysates with Rabbit anti-HtrA2 / Omi antibody (ET1609-73) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: SH-SY5Y cell lysate (20 µg/Lane) Lane 5: Mouse liver tissue lysate (40 µg/Lane) Lane 6: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 49 kDa Observed band size: 38 kDa Exposure time: 17 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-73) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-HtrA2 / Omi antibody (ET1609-73) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-73) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-HtrA2 / Omi antibody (ET1609-73) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-73) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-HtrA2 / Omi antibody (ET1609-73) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-73) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.