ET1609-74

CD44 Recombinant Rabbit Monoclonal Antibody [ST57-03]

cat.: ET1609-74

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human
Applications:	WB, IHC-P, FC
Clonality:	Monoclonal
Clone number:	ST57-03

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 82 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within human CD44 aa 150-190.
Positive control:	HeLa cell lysate, human tonsil tissue, human colon carcinoma tissue, human spleen tissue, human breast carcinoma tissue, Hela.
Subcellular location:	Cell membrane.
Recommended Dilutions: WB IHC-P FC	1:500-1:1,000 1:50-1:200 1:50-1:100
Uniprot #:	SwissProt: P16070 Human
Alternative names:	LHR BA-1 CD 44 CD44 CD44 antigen CD44 molecule (Indian blood group) CD44 molecule CD44_HUMAN CDw44 Cell surface glycoprotein CD44 chondroitin sulfate proteoglycan 8 CSPG8 ECMR-III Epican Extracellular matrix receptor III GP90 lymphocyte homing/adhesion receptor HCELL hematopoietic cell E- and L-selectin ligand Heparan sulfate proteoglycan Hermes antigen homing function and Indian blood group system HSA HUTCH-I HUTCH1 Hyaluronate receptor IN MC56 MDU2 MDU3 MGC10468 MIC4 MUTCH1 PGP-1 PGP-I PGP1 Phagocytic glycoprotein 1 Phagocytic glycoprotein I Soluble CD44

Images

	Fig1: Western blot analysis of CD44 on HeLa cell lysates with Rabbit anti-CD44 antibody (ET1609-74) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 82 kDa Observed band size: 82 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-74) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
	Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD44 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1609-74, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-CD44 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1609-74, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD44 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1609-74, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CD44 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1609-74, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-CD44 antibody (ET1609-74) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1609-74) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Flow cytometric analysis of CD44 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1609-74, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.