Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody [ST05-43]
cat.: ET1609-75
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC-P
Clonality: Monoclonal
Clone number: ST05-43
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 59 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Cytokeratin 10 aa 541-584 / 584.
Positive control: Human skin tissue lysates, Hela, human tonsil tissue, mouse skin tissue, rat skin tissue.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  ICC
  IF
  IHC-P

1:500-1:2,000
1:50-1:200
1: 500
1:50-1:200
Uniprot #: SwissProt: P13645 Human | P02535 Mouse | Q6IFW6 Rat
Alternative names: BCIE BIE CK 10 CK-10 Cytokeratin-10 EHK K10 K1C10_HUMAN Keratin 10 Keratin Keratin type i cytoskeletal 10 Keratin type I cytoskeletal 59 kDa Keratin-10 Keratin10 KPP KRT10 type I cytoskeletal 10
Images
ET1609-75_1.jpg Fig1: Western blot analysis of Cytokeratin 10 on human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-75, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1609-75_2.jpg Fig2: ICC staining of Cytokeratin 10 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-75, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1609-75_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-75, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1609-75_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Cytokeratin 10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-75, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1609-75_5.jpg Fig5: Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 10 (ET1609-75).

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 10 (ET1609-75, red) at 1/500 dilution at +4℃ overnight, washed with PBS.

Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
ET1609-75_6.jpg Fig6: Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 10 (ET1609-75).

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 10 (ET1609-75, red) at 1/500 dilution at +4℃ overnight, washed with PBS.

Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
ET1609-75_7.jpg Fig7: Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 10 (ET1609-75) and Vimentin (EM0401).

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 10 (ET1609-75, red) at 1/400 dilution and Vimentin (EM0401, green) at 1/400 dilution at +4℃ overnight, washed with PBS.

Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) and Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.