Acetyl CoA Carboxylase 1 (ACC1) Recombinant Rabbit Monoclonal Antibody [ST53-08]
cat.: ET1609-77
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | ST53-08 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 266 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ACC1 aa 382-427 / 2346. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, HEK-293 cell lysate, A549 cell lysate, C2C12 cell lysate, RAW264.7 cell lysate, C6 cell lysate, human kidney tissue, mouse placenta tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q13085 Human | Q5SWU9 Mouse | P11497 Rat |
| Alternative names: | ACAC ACACA ACACA_HUMAN ACC alpha ACC ACC-alpha ACC1 ACCA Acetyl CoA carboxylase 1 Acetyl CoA carboxylase alpha Acetyl Coenzyme A carboxylase alpha Biotin carboxylase COA1 COA2 HACC275 OTTHUMP00000164069 OTTHUMP00000164070 OTTHUMP00000164076 OTTHUMP00000240532 |
Images
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Fig1:
Western blot analysis of Acetyl CoA Carboxylase 1 (ACC1) on different lysates with Rabbit anti-Acetyl CoA Carboxylase 1 (ACC1) antibody (ET1609-77) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: HEK-293 cell lysate (20 µg/Lane) Lane 4: A549 cell lysate (20 µg/Lane) Lane 5: C2C12 cell lysate (20 µg/Lane) Lane 6: RAW264.7 cell lysate (20 µg/Lane) Lane 7: C6 cell lysate (20 µg/Lane) Predicted band size: 266 kDa Observed band size: 250/266 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-77) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Acetyl CoA Carboxylase 1 (ACC1) on different lysates with Rabbit anti-Acetyl CoA Carboxylase 1 (ACC1) antibody (ET1609-77) at 1/1,000 dilution. Lane 1: HepG2-si NT cell lysate Lane 2: HepG2-si ACC1 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 266 kDa Observed band size: 250/266 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-77) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Acetyl CoA Carboxylase 1 (ACC1) antibody (ET1609-77) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-77) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Acetyl CoA Carboxylase 1 (ACC1) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-77, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.