MSI2 Recombinant Rabbit Monoclonal Antibody [ST59-02]
cat.: ET1609-79
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC
Clonality: Monoclonal
Clone number: ST59-02
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 35 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human MSI2 aa 180-300 / 328.
Positive control: Hela, LOVO, human liver tissue, human pancreas tissue, mouse liver tissue, mouse prostate tissue, mouse heart tissue.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
1:1,000
1:50-1:200
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q96DH6 Huma | Q920Q6 Mouse
Entrez Gene: 360596 Rat
Alternative names: FLJ36569 MGC3245 Msi2 MSI2/HOXA9 fusion gene, included MSI2H MSI2H_HUMAN Musashi 2 Musashi homolog 2 Musashi RNA binding protein 2 Musashi, Drosophila, homolog of, 2 Musashi-2 RNA binding protein Musashi homolog 2 RNA-binding protein Musashi homolog 2 WD 40 repeat protein MSI2
Images
ET1609-79_1.jpg Fig1: Western blot analysis of MSI2 on different lysates with Rabbit anti-MSI2 antibody (ET1609-79) at 1/1,000 dilution.

Line 1: LOVO cell lysates
Line 2: PC-12 cell lysates

Lysates/proteins at 10 µg/Lane.

Predicted band size: 35 kDa
Observed band size: 35/37 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-79) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 5,000 dilution was used for 1 hour at room temperature.
ET1609-79_2.jpg Fig2: Western blot analysis of MSI2 on different lysates with Rabbit anti-MSI2 antibody (ET1609-79) at 1/1,000 dilution.

Lane 1: Hela-si NT cell lysate
Lane 2: Hela-si MSI2#1 cell lysate
Lane 3: Hela-si MSI2#2 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 35 kDa
Observed band size: 35 kDa

Exposure time: 60 seconds;

4-20% SDS-PAGE gel.

ET1609-79 was shown to specifically react with MSI2 in Hela-si NT cells. No bands were observed when Hela-si MSI2 samples were tested. Hela-si NT and Hela-si MSI2 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1609-79, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
ET1609-79_3.jpg Fig3: Immunocytochemistry analysis of Hela cells labeling MSI2 with Rabbit anti-MSI2 antibody (ET1609-79) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MSI2 antibody (ET1609-79) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET1609-79_4.jpg Fig4: Immunocytochemistry analysis of LOVO cells labeling MSI2 with Rabbit anti-MSI2 antibody (ET1609-79) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MSI2 antibody (ET1609-79) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET1609-79_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-MSI2 antibody (ET1609-79) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-79) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1609-79_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-MSI2 antibody (ET1609-79) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-79) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1609-79_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-MSI2 antibody (ET1609-79) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-79) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1609-79_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue with Rabbit anti-MSI2 antibody (ET1609-79) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-79) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1609-79_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-MSI2 antibody (ET1609-79) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-79) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1609-79_10.jpg Fig10: Flow cytometric analysis of Hela cells with MSI2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.