Histone H2B (mono methyl R79) Recombinant Rabbit Monoclonal Antibody [ST0430]
cat.: ET1609-9
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: IF-Cell, IHC-P
Clonality: Monoclonal
Clone number: ST0430
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 14 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Histone H2B aa 51-100 / 126.
Positive control: Mouse colon tissue, mouse testis tissue, rat colon tissue, rat testis tissue, human tonsil tissue, human breast carcinoma tissue, HepG2, Hela, MCF-7.
Subcellular location: Nucleus, Chromosome.
Recommended Dilutions:
  IF-Cell
  IHC-P
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: O60814 Human | P06899 Human | P23527 Human | P33778 Human | P57053 Human | P58876 Human | P62807 Human | Q16778 Human | Q5QNW6 Human | Q8N257 Human | Q93079 Human | Q99877 Human | Q99879 Human | Q99880 Human | P10854 Mouse | Q64475 Mouse | Q00729 Rat
Alternative names: H2B 1A H2B H2B histone family H2B2f H2B2F_HUMAN H2Ba H2Bf HIST2H2BF histone H2B histone H2B type 1 Histone H2B type 2-F MGC131639 H2BR79me
Images
ET1609-9_1.jpg Fig1: Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Histone H2B (mono methyl R79) antibody (ET1609-9) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-9) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1609-9_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Histone H2B (mono methyl R79) antibody (ET1609-9) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-9) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1609-9_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-Histone H2B (mono methyl R79) antibody (ET1609-9) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-9) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1609-9_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-Histone H2B (mono methyl R79) antibody (ET1609-9) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-9) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1609-9_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2B(mono methyl R79) antibody. Counter stained with hematoxylin.
ET1609-9_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2B(mono methyl R79) antibody. Counter stained with hematoxylin.
ET1609-9_7.jpg Fig7: ICC staining Histone H2B(mono methyl R79) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1609-9_8.jpg Fig8: ICC staining Histone H2B(mono methyl R79) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1609-9_9.jpg Fig9: ICC staining Histone H2B(mono methyl R79) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.