Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse |
Applications: | WB, IF-Cell, IF-Tissue, IHC-P |
Clonality: | Monoclonal |
Clone number: | ST0430 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 14 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human Histone H2B aa 51-100 / 126. |
Positive control: | MCF-7, Hela, HepG2, human tonsil tissue, mouse testis tissue, human breast carcinoma tissue. |
Subcellular location: | Nucleus, Chromosome. |
Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P |
1:1,000 1:50-1:200 1:50-1:200 1:50-1:200 |
Uniprot #: | SwissProt: P06899 Human | O60814 Human | P33778 Human | P23527 Human | Q64524 Mouse |
Alternative names: | H2B 1A H2B H2B histone family H2B2f H2B2F_HUMAN H2Ba H2Bf HIST2H2BF histone H2B histone H2B type 1 Histone H2B type 2-F MGC131639 H2BR79me1 |
Fig1: ICC staining Histone H2B(mono methyl R79) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig2: ICC staining Histone H2B(mono methyl R79) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: ICC staining Histone H2B(mono methyl R79) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2B(mono methyl R79) antibody. Counter stained with hematoxylin. |
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2B(mono methyl R79) antibody. Counter stained with hematoxylin. | |
Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H2B(mono methyl R79) antibody. Counter stained with hematoxylin. |