CD11a Recombinant Rabbit Monoclonal Antibody [SC56-01]
cat.: ET1610-1
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SC56-01 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 129 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CD11a aa 1,135-1,170 / 1,170. |
| Positive control: | THP-1 cell lysate, human colon tissue, human tonsil tissue, human spleen tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Tissue IHC-P IP |
1:2,000-1:5,000 1:50-1:200 1:200-1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: P20701 Human |
| Alternative names: | Lymphocyte function associated antigen, type 1, alpha subunit Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide Antigen CD11A CD 11a CD11 antigen-like family member A CD11a CD11a antigen Integrin Alpha L Integrin alpha-L Integrin gene promoter Integrin, alpha L (antigen CD11A (p180), lymphocyte function associated antigen 1; alpha polypeptide) ITAL_HUMAN Itgal ITGAL protein Leukocyte adhesion glycoprotein LFA 1 alpha chain Leukocyte adhesion glycoprotein LFA-1 alpha chain Leukocyte Adhesion Glycoprotein LFA1 Alpha Chain Leukocyte function associated molecule 1 alpha chain Leukocyte function-associated molecule 1 alpha chain LFA 1 alpha (LFA1A) LFA 1 alpha LFA 1 LFA 1A LFA-1A LFA1A Ly15 Ly21 Lymphocyte function associated antigen 1 Lymphocyte Function Associated Antigen Type 1 alpha lymphocyte function-associated antigen 1, alpha polypeptide p180 |
Images
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Fig1:
Western blot analysis of CD11a on different lysates with Rabbit anti-CD11a antibody (ET1610-1) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: K-562 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 129 kDa Observed band size: 180 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-1) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-CD11a antibody (ET1610-1) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-1) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD11a antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-1, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD11a antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-1, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
CD11a was immunoprecipitated from 0.2 mg THP-1 cell lysate with ET1610-1 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using ET1610-1 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: THP-1 cell lysate (input) Lane 2: ET1610-1 IP in THP-1 cell lysate Lane 3: Rabbit IgG instead of ET1610-1 in THP-1 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 2 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.