Anti-JNK2 antibody [SC05-85]
cat.: ET1610-11
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P, IP, FC
Clonality: Monoclonal
Clone number: SC05-85
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 46/54 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human JNK2 aa 350-390.
Positive control: SHG-44 cell lysates, Hela, MCF-7, SHG-44, mouse brain tissue.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC
  IP

1:1,000-1:5,000
1:50-1:200
1:50-1:200
1:50-1:100
Use at an assay dependent concentration.
Uniprot #: SwissProt: P45984 Human | Q9WTU6 Mouse | P49186 Rat
Alternative names: c Jun kinase 2 antibody C Jun N terminal kinase 2 antibody c-Jun N-terminal kinase 2 antibody JNK 55 antibody JNK-55 antibody JNK2 alpha antibody JNK2 antibody JNK2 beta antibody JNK2A antibody JNK2alpha antibody JNK2B antibody JNK2BETA antibody Jun kinase antibody MAP kinase 9 antibody MAPK 9 antibody Mapk9 antibody Mitogen activated protein kinase 9 antibody Mitogen-activated protein kinase 9 antibody MK09_HUMAN antibody P54a antibody p54aSAPK antibody PRKM9 antibody Protein kinase, mitogen-activated, 9 antibody SAPK alpha antibody SAPK antibody SAPK1a antibody Stress activated protein kinase 1a antibody Stress-activated protein kinase JNK2 antibody
Images
ET1610-11_1.jpg Fig1: Western blot analysis of JNK2 on SHG-44 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1610-11_2.jpg Fig2: ICC staining of JNK2 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-11, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1610-11_3.jpg Fig3: ICC staining of JNK2 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-11, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1610-11_4.jpg Fig4: ICC staining of JNK2 in SHG-44 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-11, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1610-11_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-JNK2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-11, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1610-11_6.jpg Fig6: Flow cytometric analysis of JNK2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1610-11, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.