UBC9 Recombinant Rabbit Monoclonal Antibody [SC0534]
cat.: ET1610-21
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SC0534 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 18 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human UBC9 aa 15-60 / 158. |
| Positive control: | HUVEC cell lysate, HepG2 cell lysate, Hela cell lysate, SH-SY5Y cell lysate, A431, HepG2, human kidney tissue, mouse testis tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P IP |
1:500- 1:2,000 1:50-1:200 1:50-1:200 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P63279 Human | P63280 Mouse | P63281 Rat |
| Alternative names: | C358B7.1 p18 SUMO 1 protein ligase SUMO conjugating enzyme UBC9 SUMO-conjugating enzyme UBC9 SUMO-protein ligase SUMO1 protein ligase UBC9 UBC9_HUMAN UBCE9 Ube2i Ubiquitin carrier protein 9 Ubiquitin carrier protein Ubiquitin carrier protein I Ubiquitin conjugating enzyme 9 Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9) Ubiquitin conjugating enzyme E2I (UBC9 homolog, yeast) Ubiquitin conjugating enzyme UbcE2A Ubiquitin like protein SUMO 1 conjugating enzyme Ubiquitin protein ligase E2I Ubiquitin-conjugating enzyme E2 I Ubiquitin-protein ligase I |
Images
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Fig1:
Western blot analysis of UBC9 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-21, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HUVEC cell lysate Lane 2: HepG2 cell lysate Lane 3: Hela cell lysate Lane 4: SH-SY5Y cell lysate |
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Fig2: ICC staining of UBC9 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-21, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of UBC9 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-21, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-UBC9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-21, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-UBC9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-21, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.