Anti-UBC9 antibody [SC0534]
cat.: ET1610-21
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC-P, IP, ChIP
Clonality: Monoclonal
Clone number: SC0534
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 18 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human UBC9 aa 10-60.
Positive control: HUVEC cell lysate, HepG2 cell lysate, Hela cell lysate, SH-SY5Y cell lysate, A431, HepG2, human kidney tissue, mouse testis tissue.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  IP

1:500- 1:2,000
1:50-1:200
1:50-1:200
Use at an assay dependent concentration.
Uniprot #: SwissProt: P63279 Human | P63281 Rat
Alternative names: C358B7.1 antibody p18 antibody SUMO 1 protein ligase antibody SUMO conjugating enzyme UBC9 antibody SUMO-conjugating enzyme UBC9 antibody SUMO-protein ligase antibody SUMO1 protein ligase antibody UBC9 antibody UBC9_HUMAN antibody UBCE9 antibody Ube2i antibody Ubiquitin carrier protein 9 antibody Ubiquitin carrier protein antibody Ubiquitin carrier protein I antibody Ubiquitin conjugating enzyme 9 antibody Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9) antibody Ubiquitin conjugating enzyme E2I (UBC9 homolog, yeast) antibody Ubiquitin conjugating enzyme UbcE2A antibody Ubiquitin like protein SUMO 1 conjugating enzyme antibody Ubiquitin protein ligase E2I antibody Ubiquitin-conjugating enzyme E2 I antibody Ubiquitin-protein ligase I antibody
Images
ET1610-21_1.jpg Fig1: Western blot analysis of UBC9 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-21, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HUVEC cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Hela cell lysate
Lane 4: SH-SY5Y cell lysate
ET1610-21_2.jpg Fig2: ICC staining of UBC9 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-21, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1610-21_3.jpg Fig3: ICC staining of UBC9 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-21, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1610-21_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-UBC9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-21, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1610-21_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-UBC9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-21, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.