p95 / NBS1 Recombinant Rabbit Monoclonal Antibody [SC05-68]
cat.: ET1610-26
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SC05-68 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 85 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human p95 NBS1 aa 680-730. |
| Positive control: | HeLa cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse testis tissue lysate, human large intestine tissue. |
| Subcellular location: | Nucleus, Chromosome. |
| Recommended Dilutions:
WB IF-Tissue IHC-P IP |
1:1,000 1:50-1:200 1:100 1-2μg/sample |
| Uniprot #: | SwissProt: O60934 Human | Q9R207 Mouse | Q9JIL9 Rat |
| Alternative names: | AT V1 AT V2 ATV Cell cycle regulatory protein p95 FLJ10155 MGC87362 Nbn NBN_HUMAN NBS 1 NBS NBS1 Nibrin Nijmegen breakage syndrome 1 (nibrin) Nijmegen breakage syndrome Nijmegen breakage syndrome protein 1 p95 p95 protein of the MRE11/RAD50 complex |
Images
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Fig1:
Western blot analysis of p95 / NBS1 on different lysates with Rabbit anti-p95 / NBS1 antibody (ET1610-26) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: NIH/3T3 cell lysate (20 µg/Lane) Lane 3: PC-12 cell lysate (20 µg/Lane) Lane 4: Mouse testis tissue lysate (40 µg/Lane) Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-26) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human large intestine tissue with Rabbit anti-p95 / NBS1 antibody (ET1610-26) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-26) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
p95 / NBS1 was immunoprecipitated from 0.2 mg HeLa cell lysate with ET1610-26 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1610-26 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: ET1610-26 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of ET1610-26 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.