Anti-p95 NBS1 antibody [SC05-68]
cat.: ET1610-26
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IP, FC, IHC-P, ChIP
Clonality: Monoclonal
Clone number: SC05-68
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A purified.
Molecular weight: 95 kDa
Isotype: IgG
Immunogen: recombinant protein
Positive control: A431, Hela, human lung tissue.
Subcellular location: Nucleus, Chromosome.
Recommended Dilutions:
  WB
  ICC/IF
  FC
  IHC-P

1:1,000
1:50-1:200
1:50-1:100
1:50
Uniprot #: O60934(Human) Q9R207(Mouse) Q9JIL9(Rat)
Alternative names: AT V1 antibody AT V2 antibody ATV antibody Cell cycle regulatory protein p95 antibody FLJ10155 antibody MGC87362 antibody Nbn antibody NBN_HUMAN antibody NBS 1 antibody NBS antibody NBS1 antibody Nibrin antibody Nijmegen breakage syndrome 1 (nibrin) antibody Nijmegen breakage syndrome antibody Nijmegen breakage syndrome protein 1 antibody p95 antibody p95 protein of the MRE11/RAD50 complex antibody
Images
ET1610-26_1.jpg Fig1: Western blot analysis of Nibrin on human lung lysates using anti-p95 NBS1 antibody at 1/1,000 dilution.
ET1610-26_2.jpg Fig2: ICC staining p95 NBS1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1610-26_3.jpg Fig3: ICC staining p95 NBS1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1610-26_4.jpg Fig4: Flow cytometric analysis of Hela cells with p95 NBS1antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.