p95 / NBS1 Recombinant Rabbit Monoclonal Antibody [SC05-68]
cat.: ET1610-26
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IP, FC, IHC-P
Clonality: Monoclonal
Clone number: SC05-68
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 85 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human p95 NBS1 aa 680-730.
Positive control: HeLa cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse testis tissue lysate, Hela, A431, human large intestine tissue.
Subcellular location: Nucleus, Chromosome.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  FC
  IHC-P
1:1,000
1:50-1:200
1:50-1:200
1:50-1:100
1:100
Uniprot #: SwissProt: O60934 Human | Q9R207 Mouse | Q9JIL9 Rat
Alternative names: AT V1 AT V2 ATV Cell cycle regulatory protein p95 FLJ10155 MGC87362 Nbn NBN_HUMAN NBS 1 NBS NBS1 Nibrin Nijmegen breakage syndrome 1 (nibrin) Nijmegen breakage syndrome Nijmegen breakage syndrome protein 1 p95 p95 protein of the MRE11/RAD50 complex
Images
ET1610-26_1.jpg Fig1: Western blot analysis of p95 / NBS1 on different lysates with Rabbit anti-p95 / NBS1 antibody (ET1610-26) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: NIH/3T3 cell lysate (20 µg/Lane)
Lane 3: PC-12 cell lysate (20 µg/Lane)
Lane 4: Mouse testis tissue lysate (40 µg/Lane)

Predicted band size: 85 kDa
Observed band size: 85 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-26) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1610-26_2.jpg Fig2: ICC staining of p95 / NBS1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-26, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1610-26_3.jpg Fig3: ICC staining of p95 / NBS1 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-26, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1610-26_4.jpg Fig4: Flow cytometric analysis of p95 / NBS1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1610-26, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
ET1610-26_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human large intestine tissue with Rabbit anti-p95 / NBS1 antibody (ET1610-26) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-26) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.